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AB0100

Sigma-Aldrich

Alkaline Phosphatase Blue Microwell Substrate

alkaline phosphatase substrate, solution

Sinônimo(s):

5-bromo-4-chloro-3-indolyl phosphate analog / NBT substrate for microwell assays

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About This Item

Código UNSPSC:
12352204
NACRES:
NA.83

product name

Alkaline Phosphatase Blue Microwell Substrate, sufficient for 400 mL working substrate

forma

solution

Nível de qualidade

200

uso

sufficient for 400 mL working substrate

temperatura de armazenamento

2-8°C

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Descrição geral

Alkaline phosphatase blue microwell substrate, a two-component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require an insoluble reaction product.

Aplicação

Prior to reaction with alkaline phosphatase, the BCIP® reagent is colorless to faint blue solution, and the Nitro BT reagent is a yellow solution. The two component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require a precipitation reaction product.

forma física

A 2 component kit sufficient for preparing 400 mL of working substrate.
Supplied as a 2 component buffered alkaline phosphatase substrate containing Bromo-Chloro-Indolylphosphate (BCIP) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)

Informações legais

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

10 - Combustible liquids

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Artigos

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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