A6362

Alpha-lytic protease

• Sinônimo(s): Alpha-lytic endopeptidase, alphaLP
• Número da licença da enzima: 3.4.21.12 (BRENDA, IUBMB)
• Código UNSPSC: 12352204
• NACRES: NA.54

Propriedades

• fonte biológica: bacterial
• Nível de qualidade: 100
• Formulário: liquid
• atividade específica: ≥0.0005 U/mg
• peso molecular: 19,860 Da
• pH ideal: 5.0(storage); 7.5(activity)
• pI: 9.69
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C

Descrição

Descrição geral

Alpha-lytic protease (aLP) is an alternative specificity protease for proteomics applications. This protease cleaves after T, A, S, and V residues. It generates peptides of similar average length as trypsin.

aLP was first isolated from the myxobacterium Lysobacter enzymogenes. The pro-form of aLP is 397 amino acids long. In its mature form, aLP is 198 amino acids long. Its tertiary structural core resembles those of pancreatic serine proteases.

Crystal structure studies of aLP have been reported. Several studies are available on the active site and catalytic mechanism of aLP. The role of the pro-region in the activation, secretion and folding of aLP has been studied.

The activity of aLP in the presence of various solution components is as follows:

• 0.1% sodium deoxycholate: ~1.75-fold enhanced activity
• 1.0% sodium deoxycholate: ~60% activity
• 0.1% SDS: ~50% activity
• 1.0% SDS: ~40% activity
• 1 M guanidine HCl: ~20% activity
• 4 M guanidine HCl: ~1% activity (essentially inactivated)

Definição da unidade

One unit will produce one mmole of p-nitroaniline per minute from N-succinyl-Ala-Ala-Ala-PNA at 25 °C at pH 7.5

forma física

Supplied as a solution in 10 mM sodium acetate buffer, pH 5.0.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable