A5168
Adenosine Deaminase from bovine spleen
Type IX, ammonium sulfate suspension, 150-200 units/mg protein
Sinônimo(s):
Adenosine aminohydrolase
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Produtos recomendados
fonte biológica
bovine spleen
Nível de qualidade
tipo
Type IX
Formulário
ammonium sulfate suspension
atividade específica
150-200 units/mg protein
peso molecular
32.5-33 kDa
nº de adesão UniProt
atividade externa
5′-AMP deaminase ≤0.01%
alkaline phosphatase ≤0.01%
guanase ≤0.01%
nucleoside phosphorylase ≤0.1%
temperatura de armazenamento
2-8°C
Informações sobre genes
cow ... ADA(280712)
Procurando produtos similares? Visita Guia de comparação de produtos
Descrição geral
Adenosine deaminase/ADA is an enzyme, that is mainly present in lymphocytes and monocytes.
Molecular weight: 32.5-33 kDa
pI: 4.85
pI: 4.85
Aplicação
Adenosine Deaminase from bovine spleen has been used to metabolize adenosine triphosphate (ATP) and adenosine to an inactive form (inosine). It has also been used to as an additive in KRH (Krebs−Ringer solution buffered with Hepes, containing CaCl2) medium in order to remove exogenous adenosine for cyclic adenosine monophosphate (cAMP) assay.
Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.
Ações bioquímicas/fisiológicas
Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.
Adenosine deaminase/ADA can be used as an important marker to identify tuberculous pleural effusion. The activity of ADA is usually high in liver disease, tuberculosis, typhoid, infective mononucleosis and several malignancies.
Definição da unidade
One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.
forma física
Suspension in 3.2 M (NH4)2SO4, 0.01 M potassium phosphate, pH 6.0
Nota de análise
Protein determined by biuret.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Escolha uma das versões mais recentes:
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
The diagnostic value of adenosine deaminase activity in pulmonary tuberculosis: Comparison between sputum and serum
Atta S, et al.
The Egyptian journal of chest diseases and tuberculosis, 64(1), 103-107 (2015)
Paracrine stimulation of surfactant secretion by extracellular ATP in response to mechanical deformation
Patel A S, et al.
American Journal of Physiology. Lung Cellular and Molecular Physiology, 289(3), L489-L496 (2005)
Reduced PDE4 expression and activity contributes to enhanced catecholamine-induced cAMP accumulation in adipocytes from FOXC2 transgenic mice
Gronning L M, et al.
Febs Letters, 580(17), 4126-4130 (2006)
Ying Cheng et al.
Diabetes, 59(1), 17-25 (2009-10-17)
White adipose tissue (WAT) and brown adipose tissue (BAT) play distinct roles in adaptation to changes in nutrient availability, with WAT serving as an energy store and BAT regulating thermogenesis. We previously showed that mice maintained on a leucine-deficient diet
George R Thompson et al.
Chest, 143(3), 776-781 (2012-11-29)
In a patient with positive serum serology for coccidioidomycosis, the differential diagnosis of concurrent pleural effusions can be challenging. We, therefore, sought to clarify the performance characteristics of biochemical, serologic, and nucleic-acid-based testing in an attempt to avoid invasive procedures.
Artigos
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica