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Merck
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Key Documents

10102121001

Roche

Adenosine Deaminase (ADA)

from calf intestine

Sinônimo(s):

ADA, Adenosine Deaminase

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About This Item

Número da licença da enzima:
Código UNSPSC:
12352204

fonte biológica

bovine intestine

Nível de qualidade

forma

suspension

atividade específica

~200 units/mg protein (At 25 °C with adenosine as the substrate.)

embalagem

pkg of 1 mL (10 mg)

fabricante/nome comercial

Roche

técnica(s)

activity assay: suitable

cor

white

pH ideal

7.0-7.4

solubilidade

water: miscible

adequação

suitable for chemiluminescence

nº de adesão NCBI

nº de adesão UniProt

aplicação(ões)

life science and biopharma

atividade externa

AMP-deaminase <0.01%
AP <0.01% (with pre incubation)
Guanase <0.01%
NP <0.01%

temperatura de armazenamento

2-8°C

Informações sobre genes

bovine ... ADA(280712)

Descrição geral

Adenosine aminohydrolase.
Adenosine deaminase (ADA) is a zinc metalloenzyme.

Aplicação

Chemiluminescent adenosine assay.
Deamination of adenosine analogs to the corresponding inosine analogs
Adenosine deaminase (ADA) has been used in lipolysis assay along with other reagents.

Ações bioquímicas/fisiológicas

Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.

Qualidade

Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each

forma física

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Small particles are normal. Before using stir up the precipitate and proceed.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

No data available

Ponto de fulgor (°C)

No data available


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Cultured astrocytes do not release adenosine during hypoxic conditions.
Fujita T, et al.
Journal of Cerebral Blood Flow and Metabolism, 32(1), e1-e7 (2012)
Mi-Jeong Lee et al.
Methods in enzymology, 538, 49-65 (2014-02-18)
Obesity is reaching epidemic proportions so there is growing interest in the mechanisms that regulates adipose tissue development and function. Although murine adipose cell lines are useful for many mechanistic studies, primary human adipose stromal cells (ASCs), which can be
Adenosine deaminase from calf intestinal mucosa.
C A ZITTLE
The Journal of biological chemistry, 166(2), 499-503 (1946-12-01)
Sonia Muñoz-López et al.
Frontiers in endocrinology, 13, 1007801-1007801 (2022-11-22)
Despite great efforts, effective treatment against cancer has not yet been found. However, natural compounds such as the polyphenol resveratrol have emerged as promising preventive agent in cancer therapy. The mode of action of resveratrol is still poorly understood, but
D J Porter et al.
The Journal of biological chemistry, 268(4), 2480-2485 (1993-02-05)
The protein fluorescence of adenosine deaminase (ADA) was perturbed during the deamination of adenosine and four analogues of adenosine. The kinetics for the approach to the steady-state during turnover were monitored by fluorescence changes associated with formation of enzymatic intermediates.

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