A4213
Monoclonal Anti-Maltose Binding Protein−Peroxidase antibody produced in mouse
clone MBP-17, purified immunoglobulin, lyophilized powder
Sinônimo(s):
Monoclonal Anti-Maltose Binding Protein, Anti-MBP
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.46
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
conjugado
peroxidase conjugate
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
MBP-17, monoclonal
Formulário
lyophilized powder
embalagem
vial of 0.5 mL
técnica(s)
western blot: 1:1,000 using purified, recombinant MBP
Isotipo
IgG1
temperatura de armazenamento
2-8°C
modificação pós-traducional do alvo
unmodified
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Descrição geral
Monoclonal Anti-Maltose Binding Protein (MBP) (mouse IgG1 isotype) is derived from the MBP-17 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified recombinant MBP fusion protein. Maltose Binding Protein (MBP) is a periplasmic protein, which is specific to bacteria. It has maltodextrin binding site and binds to linear and cyclic maltodextrins. The mature MBP is produced from the pre-protein containing 26 amino acids, as signal peptide.
The antibody recognizes native as well as denatured-reduced forms of purified MBP and MBP fusion proteins.
Imunogênio
Purified, recombinant MBP fusion protein
Aplicação
Endogenous intracellular levels of MBP in salmonella cells were determined by western blot analysis using a monoclonal anti-MBP antibody.
Monoclonal Anti-Maltose Binding Protein Peroxidase antibody produced in mouse has been used in:
- immunoblotting
- dot blot
- enzyme-linked immunosorbent assay (ELISA)
Ações bioquímicas/fisiológicas
Maltose Binding Protein (MBP) is often used to tag recombinant proteins to enhance the yield and facilitate the purification of the fusion product. Additionally, MBP tags can also improve the immunogenicity of the tagged protein. Thus, MBP can also be used as a carrier protein for vaccinations.
Maltose Binding Protein (MBP) mediates uptake of maltose. MBP is a substrate for chaperone secB. It has been reported that the addition of a maltose binding protein (MBP) tag creates a stable fusion product that does not appear to interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product.
forma física
Lyophilized in 0.01 M sodium phosphate buffered saline containing 1% bovine serum albumin and 0.05% MIT.
Nota de preparo
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Reconstituição
The antibody conjugate should be reconstituted with 0.5 mL deionized water.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Skin Sens. 1
Código de classe de armazenamento
13 - Non Combustible Solids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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P Bellot et al.
Veterinary immunology and immunopathology, 152(1-2), 101-108 (2012-10-20)
Maltose binding protein (MBP) is often fused to a relevant protein to improve its yield and facilitate its purification, but MBP can also enhance the immunogenicity of the fused proteins. Recent data suggest that MBP may potentiate antigen-presenting functions in
Effect of signal peptide on the stability and folding kinetics of maltose binding protein.
Beena K, et al.
Biochemistry, 43(12), 3608-3619 (2004)
The 2.3-A resolution structure of the maltose-or maltodextrin-binding protein, a primary receptor of bacterial active transport and chemotaxis.
Spurlino JC, et al.
The Journal of Biological Chemistry, 266(8), 5202-5219 (1991)
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