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A2404

Sigma-Aldrich

Alcohol Oxidase solution from Pichia pastoris

buffered aqueous solution, 10-40 units/mg protein (biuret)

Sinônimo(s):

Alcohol:oxygen oxidoreductase

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About This Item

Número CAS:
9073-63-6
Número da licença da enzima:
1.1.3.13 (BRENDA, IUBMB)
Número MDL:
MFCD00130452
Código UNSPSC:
12352204
NACRES:
NA.54

forma

buffered aqueous solution

Nível de qualidade

200

atividade específica

10-40 units/mg protein (biuret)

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

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Descrição geral

Alcohol oxidase (AOX) is a homo-octamer, which has a molecular weight of 600-kDa. It has eight flavin adenine dinucleotide (FAD) cofactors. It belongs to the glucose-methanol-choline (GMC) oxidoreductase family.

Aplicação

Alcohol Oxidase may be used to study protein translocation into peroxisomes. Product A2404 is from Pichia pastoris. It has been used for the bacterial expression and immunological verification of Hv-p68 cDNA clones .
Alcohol Oxidase solution from Pichia pastoris has been used in cotton pectin methylesterase (PME) enzyme assay. It has also been used to immobilize onto polyaniline (PANI) film as a visual biosensor in dip-stick format for the determination of ethanol in fermented beverage samples.

Ações bioquímicas/fisiológicas

Alcohol oxidase catalyzes the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes. It has the highest affinity for methanol. Alcohol oxidase is involved in the metabolism of methanol, which is important for yeasts that utilize methanol as their sole energy source. Alcohol oxidase is primarily found in the peroxisome but may be present in the cytoplasm as well.

Definição da unidade

One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C.

forma física

Solution in 30% sucrose with 0.1 M phosphate buffer at pH 8.0

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

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Xuan Wang et al.
Journal of experimental botany, 71(9), 2629-2640 (2020-02-02)
De-esterification of homogalacturonan (HG) is thought to stiffen pectin gels and primary cell walls by increasing calcium cross-linking between HG chains. Contrary to this idea, recent studies found that HG de-esterification correlated with reduced stiffness of living tissues, measured by
Pectin methylesterase and pectin remodelling differ in the fibre walls of two gossypium species with very different fibre properties
Liu Q, et al.
PLoS ONE, 8(6), e65131-e65131 (2013)
Structure of alcohol oxidase from Pichia pastoris by cryo-electron microscopy
Vonck J, et al.
PLoS ONE, 11(7), e0159476-e0159476 (2016)
Christina Dorado et al.
Biology, 10(12) (2021-12-25)
Florida citrus production has declined 75% due to Huanglongbing (HLB), a disease caused by the pathogenic bacterium Candidatus Liberibacter asiaticus (CLas). Methods to combat CLas are costly and only partially effective. The cross-compatible species Poncirus trifoliata and some of its
A simple visual ethanol biosensor based on alcohol oxidase immobilized onto polyaniline film for halal verification of fermented beverage samples
Kuswandi B, et al.
Sensors, 14(2), 2135-2149 (2014)
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Protocolos

Enzymatic Assay of Alcohol Oxidase (EC 1.1.3.13)

This procedure may be used for all Alcohol Oxidase products.

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