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79760

Sigma-Aldrich

Ftaldialdeído

for fluorescence, ≥99.0% (HPLC)

Sinônimo(s):

Dicarboxaldeído o-ftálico, o-Ftalaldeído, Benzeno-1,2-dicarboxaldeído, OPA

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About This Item

Fórmula empírica (Notação de Hill):
C8H6O2
Número CAS:
643-79-8
Peso molecular:
134.13
Beilstein:
878317
Número CE:
211-402-2
Número MDL:
MFCD00003335
Código UNSPSC:
12352201
ID de substância PubChem:
57652816
NACRES:
NA.32

grau

for fluorescence

Nível de qualidade

100

Ensaio

≥99.0% (HPLC)

forma

solid

Impurezas

≤0.5% phthalic acid

pf

54-56 °C

fluorescência

λex 334 nm; λem 455 nm (Thiol Adduct)
λex 340 nm; λem 450 nm in reaction buffer (with glycine)

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

O=Cc1ccccc1C=O

InChI

1S/C8H6O2/c9-5-7-3-1-2-4-8(7)6-10/h1-6H

chave InChI

ZWLUXSQADUDCSB-UHFFFAOYSA-N

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Descrição geral

Phthaldialdehyde, also known as o-Phthalaldehyde or OPA, is a pre-column (HPLC) derivatization reagent. OPA reacts with primary amino acids (AA) except cystine to form highly fluorescent adducts. The fluorescence of the derivatives is monitored at excitation and emission wavelengths of 345nm and 455nm, respectively. The HPLC technique efficiently separates the OPA-AA derivatives.

Aplicação

Phthaldialdehyde or OPA determines amino acids in proteins from animal tissue and food samples. Quantification of amino acids adducts of tyramine and phenylethylamine produced by lactic acid bacteria used OPA as the pre-column derivative reagent for HPLC. Intracellular forms of glutathione (GSH/GSSG) are investigated using o-Phthalaldehyde as the pre-column fluorescent agent.

Ações bioquímicas/fisiológicas

O ftaldialdeído pode ser usado para a derivatização pré-coluna de aminoácidos para separação por cromatografia líquida de alto desempenho (CLAE). Ele também pode ser usado para medições citométricas de fluxo de grupos tiol de proteínas.

Palavra indicadora

Danger

Frases de perigo

H301,H314,H317,H335,H410

Classificações de perigo

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

269.6 °F - closed cup

Ponto de fulgor (°C)

132 °C - closed cup

Equipamento de proteção individual

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificados de análise (COA)

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International journal of food microbiology, 115(3), 364-368 (2007-02-20)
The ability of wine lactic acid bacteria to produce tyramine and phenylethylamine was investigated by biochemical and genetic methods. An easy and accurate plate medium was developed to detect tyramine-producer strains, and a specific PCR assay that detects the presence
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Sphingolipid ceramide N-deacylase (SCDase) catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. While SCDases show great value for the enzymatic synthesis of glycosphingolipids, they are relatively poorly characterized enzymes. In this work, the enzymatic
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This study aimed to assess the effects of physically demanding work - measured as energy expenditure (EE) during occupational physical activities (OPA) - on risk of acute myocardial infarction (AMI) among men with and without preexisting ischemic heart disease (IHD).
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