65497

TRIS acetate-EDTA Buffer solution

for Northern and Southern blotting, solution

• Código UNSPSC: 12161700

Propriedades

• grau: Molecular Biology; for molecular biology
• linha de produto: BioReagent
• Formulário: solution
• Impurezas: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• pH: 8.3±0.2
• densidade: 1.00 g/mL at 20 °C
• temperatura de armazenamento: room temp

Descrição

Descrição geral

TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but dsDNA runs faster with TAE buffer. This concentrate can be easily diluted to 0.5x before use (with molecular biology grade water).

Aplicação

Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. Especially useful for dsDNA separations.

Componentes

The TAE liquid buffer is a 1x solution that contains 0.04M TRIS acetate and 0.001M EDTA in water.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves