63689

2-Mercaptoetanol

BioUltra, for molecular biology, ≥99.0% (GC)

• Sinônimo(s): ββ-Mercaptoetanol, 2-Hidroxietilmercaptano, BME, Tioetilenoglicol
• Fórmula linear: HSCH₂CH₂OH
• Número CAS: 60-24-2
• Peso molecular: 78.13
• Beilstein: 773648
• Número CE: 200-464-6
• Número MDL: MFCD00004890
• Código UNSPSC: 12161504
• ID de substância PubChem: 57651953
• NACRES: NA.25

Propriedades

• grau: Molecular Biology; for molecular biology
• Nível de qualidade: 200
• densidade de vapor: 2.69 (vs air)
• pressão de vapor: 1 mmHg ( 20 °C)
• linha de produto: BioUltra
• Ensaio: ≥99.0% (GC)
• Formulário: liquid
• Lim. expl.: 18 %
• adequação da reação: reagent type: reductant
• concentração: 14.3 M (pure liquid)
• técnica(s): RNA extraction: suitable; protein extraction: suitable
• Impurezas: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• índice de refração: n20/D 1.500 (lit.); n20/D 1.501
• pH: 4.5-6 (20 °C, 500 g/L)
• p.e.: 157 °C (lit.)
• solubilidade: H₂O: 0.5 M at 20 °C, clear, colorless
• densidade: 1.114 g/mL at 25 °C (lit.)
• traços de cátion: Al: ≤0.5 mg/kg; Ba: ≤0.1 mg/kg; Bi: ≤0.1 mg/kg; Ca: ≤0.5 mg/kg; Cd: ≤0.05 mg/kg; Co: ≤0.02 mg/kg; Cr: ≤0.02 mg/kg; Cu: ≤0.02 mg/kg; Fe: ≤0.1 mg/kg; K: ≤0.5 mg/kg; Li: ≤0.1 mg/kg; Mg: ≤0.1 mg/kg; Mn: ≤0.02 mg/kg; Mo: ≤0.1 mg/kg; Na: ≤1 mg/kg; Ni: ≤0.05 mg/kg; Pb: ≤0.1 mg/kg; Sr: ≤0.1 mg/kg; Zn: ≤0.1 mg/kg
• cadeia de caracteres SMILES: OCCS
• λ: 0.5 M in H₂O
• Absorção UV: λ: 260 nm A_max: 1.5; λ: 280 nm A_max: 0.3
• temperatura de armazenamento: 2-8°C
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• chave InChI: DGVVWUTYPXICAM-UHFFFAOYSA-N

Descrição

Aplicação

2-Mercaptoethanol has been used

• in the protein extraction buffer prepared for leaf samples.
• as a supplement in cell culture media.
• in the procedure of RNA extraction.

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Outras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds

Informações de segurança

• Pictogramas: GHS06,GHS08,GHS05,GHS09
• Palavra indicadora: Danger
• Frases de perigo: H301 + H331,H310,H315,H317,H318,H361f,H373,H410
• Declarações de precaução: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Classificações de perigo: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• Órgãos-alvo: Liver,Heart
• Código de classe de armazenamento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): 165.2 °F - closed cup
• Ponto de fulgor (°C): 74 °C - closed cup
• Equipamento de proteção individual: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter