56575
GenElute™-LPA
Neutral carrier for ethanol precipitation
Sinônimo(s):
linear polyacrylamide
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.52
Produtos recomendados
Nível de qualidade
temperatura de armazenamento
2-8°C
Aplicação
GenElute™-LPA has been used to:
- extract DNA from cyanobacterial and algal cultures
- precipitate genomic DNA
- extract and purify DNA from snap-frozen uveal melanoma tissues
- to extract RNA from isolated CD4+ cells
Ações bioquímicas/fisiológicas
The presence of LPA during ethanol precipitation results in complete recovery of fragments larger than 20 base pairs, whereas most of the DNA is lost if no carrier is used. Very short DNA fragments, less than 20 base pairs, do not coprecipitate with LPA, allowing separation of labeled DNA from unreacted nucleotides by precipitation after the labeling reaction.
Embalagem
GenElute LPA is tested for nucleases, and is supplied in nuclease-free water as a 25 mg/ml solution.
Princípio
GenElute LPA Linear Polyacrylamide is an efficient neutral carrier for precipitating picogram amounts of nucleic acids with ethanol. The nucleic acid precipitate can be collected simply by centrifugation. LPA offers several advantages for recovering DNA or studying DNA-protein interactions, relative to other carriers, such as tRNA or glycogen. tRNA interferes with DNA during phosphorylation with polynucleotide kinase and glycogen competes with protein in DNA-protein interaction studies. In contrast, LPA is completely inert. LPA is synthesized chemically and, therefore, is not contaminated with biological material. The precipitate is visible immediately upon addition of LPA, thus eliminating wait time and low temperature incubation.
Informações legais
GenElute is a trademark of Sigma-Aldrich Co. LLC
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves
Certificados de análise (COA)
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