A2917

Acrylamide/Bis-acrylamide

for molecular biology, BioReagent, 19:1 (ratio)

• Sinônimo(s): Acrylamide/Bisacrylamide, 19:1 (5% crosslinker), Polyacrylamide solution
• Número MDL: MFCD00080848
• Código UNSPSC: 41105319
• ID de substância PubChem: 329770804
• NACRES: NA.25

Propriedades

• grau: for molecular biology
• linha de produto: BioReagent
• forma: powder
• proporção de alimentação: 19:1 (ratio)
• atividade externa: DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
• cadeia de caracteres SMILES: NC(=O)C=C.C=CC(=O)NCNC(=O)C=C
• InChI: 1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
• chave InChI: OCQZXMCGTAWGEQ-UHFFFAOYSA-N

Descrição

Descrição geral

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Aplicação

Acrylamide/Bis-acrylamide has been used:

• in the preparation of urea polyacrylamide gel during a modified cross-linking affinity purification protocol (cCLAP)
• in microsatellite genotyping and nonfluorescent elastin (ELN) (GT)n polymorphism genotyping
• in polyacrylamide gel for separation of proteins in SDS-PAGE for western blotting

Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Características e benefícios

Eliminates the need to weigh toxic acrylamide and bis-acrylamide.

Reconstituição

Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents.

To make up 100 ml of A2917, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

Informações de segurança

• Pictogramas: GHS06,GHS08
• Palavra indicadora: Danger
• Frases de perigo: H301,H312 + H332,H315,H317,H319,H340,H350,H361fd,H372
• Declarações de precaução: P202 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Classificações de perigo: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral
• Órgãos-alvo: Peripheral nervous system
• Código de classe de armazenamento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges