19899

CHAPS 100 mM solution

• Sinônimo(s): CHAPS solution
• Fórmula empírica (Notação de Hill): C₃₂H₅₈N₂O₇S
• Número CAS: 75621-03-3
• Peso molecular: 614.88
• Número MDL: MFCD00012116
• Código UNSPSC: 12161902
• ID de substância PubChem: 57647807
• NACRES: NA.25

Propriedades

• descrição: zwitterionic
• Nível de qualidade: 100
• Formulário: liquid
• peso molecular: 614.88 g/mol
• concentração: 100 mM in H₂O
• técnica(s): protein quantification: suitable
• CMC: 8 mM
• temperatura de transição: cloud point >100 °C
• densidade: 1.01 g/mL at 20 °C
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
• InChI: 1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
• chave InChI: UMCMPZBLKLEWAF-RFCNGIAKSA-N

Descrição

Descrição geral

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) is a biocompatible surfactant. It is a derivative of cholic acid comprising a hydrophobic cholesterol-like skeleton possessing three hydroxyl groups and a zwitterionic aliphatic tail.

Aplicação

CHAPS 100 mM solution has been used as a component of lysis buffer for the extraction of proteins from goat endometrial epithelial cells (EECs) and liver tissue.

Ações bioquímicas/fisiológicas

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) exhibits the properties of both sulfobetaine-type surfactants and bile salts. It is involved in the protein extraction process for proteomic analysis and reconstitution of membrane proteins into lipid vesicles (proteoliposomes). CHAPS is broadly used in biochemistry for membrane protein and receptor isolation and purification. It is conveniently used in pharmaceuticals and medicines due to its biocompatibility, noncharged character, nondenaturing, and disaggregating properties.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves