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Documentos Principais

850500P

Avanti

CHAPS

Avanti Research - A Croda Brand

Sinônimo(s):

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

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About This Item

Fórmula empírica (Notação de Hill):
C32H58N2O7S
Número CAS:
Peso molecular:
614.88
Número MDL:
Código UNSPSC:
12161902
NACRES:
NA.25

Ensaio

>99% (TLC)

Formulário

powder

peso molecular

614.88 g/mol

embalagem

pkg of 1 × 1 g (850500P-1g)

fabricante/nome comercial

Avanti Research - A Croda Brand

técnica(s)

protein quantification: suitable

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

C[C@@]12C(CC[C@@H]2[C@@H](CCC(NCCC[N+](C)(CCCS(=O)([O-])=O)C)=O)C)C3[C@H](O)C[C@]4([H])C[C@H](O)CC[C@]4(C)C3C[C@@H]1O

InChI

1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1

chave InChI

UMCMPZBLKLEWAF-BCTGSCMUSA-N

Descrição geral

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry. Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150; aggregation number = 10) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.

Aplicação

CHAPS is suitable to prepare bicelles and to study the processes of liposome solubilization and reconstitution of transport proteins.

Embalagem

20 mL Clear Glass Screw Cap Vial (850500P-1g)

Informações legais

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Pictogramas

Exclamation mark

Palavra indicadora

Warning

Frases de perigo

Declarações de precaução

Classificações de perigo

Acute Tox. 4 Oral

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Liposome solubilization and membrane protein reconstitution using Chaps and Chapso
Cladera J, et al.
European Journal of Biochemistry, 243(3), 798-804 (1997)
Bilayer membrane modulation of membrane type 1 matrix metalloproteinase (MT1-MMP) structure and proteolytic activity
Cerofolini L, et al.
Scientific reports, 6, 29511-29511 (2016)
Functional reconstitution of the nicotinic acetylcholine receptor by CHAPS dialysis depends on the concentrations of salt, lipid, and protein
Schuerholz T, et al.
Biochemistry, 31(21), 5067-5077 (1992)
Stuart J Cordwell
Methods in molecular biology (Clifton, N.J.), 424, 139-146 (2008-03-29)
Reproducible techniques for the prefractionation of proteins prior to two-dimensional gel electrophoresis (2-DE) are essential for increasing the number of unique proteins that can be identified and assayed following biological experimentation. A simple and robust technique for separating highly soluble
Erik F Kot et al.
Langmuir : the ACS journal of surfaces and colloids, 34(11), 3426-3437 (2018-02-28)
Isotropic phospholipid bicelles are one of the most prospective membrane mimetics for the structural studies of membrane proteins in solution. Recent works provided an almost full set of data regarding the properties of isotropic bicelles; however, one major aspect of

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