19083
Phalloidin–Atto 550
suitable for fluorescence
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.32
Produtos recomendados
Ensaio
≥90% (HPLC)
Nível de qualidade
forma
solid
peso molecular
Mw 1476 g/mol
fabricante/nome comercial
ATTO-TEC GmbH
λ
in methanol
Absorção UV
λ: 552-558 nm Amax
adequação
suitable for fluorescence
temperatura de armazenamento
−20°C
Categorias relacionadas
Descrição geral
Atto 550 is a new label with high molecular absorption (120.000) and quantum yield (0.80) as well as sufficient stoke′s shift (excitation maximum 554 nm, emission maximum 576 nm). Atto 550 can be used with similar excitation source and fluorescence filters as Cy3 TM and is characterized by a high photostability.Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10–8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours.
Aplicação
Fluorescent conjugates of phalloidin, rhodamine-phalloidin staining reagents, such as Phalloidin–Atto 550 are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.
Informações legais
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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