18507
Atto 565 maleimide
BioReagent, suitable for fluorescence
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About This Item
Fórmula empírica (Notação de Hill):
C37H37ClN4O10
Peso molecular:
733.16
Número MDL:
Código UNSPSC:
12352111
NACRES:
NA.32
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linha de produto
BioReagent
Nível de qualidade
Ensaio
>90.0% (HPLC)
Formulário
powder
fabricante/nome comercial
ATTO-TEC GmbH
transmitância
254 nm
565 nm
fluorescência
λex 563 nm; λem 592 nm in 0.1 M phosphate pH 7.0
λ
(ethanol with 0.1% trifluoroacetic acid)
Absorção UV
λ: 560-566 nm Amax
adequação
corresponds for coupling to thiols
suitable for fluorescence
temperatura de armazenamento
−20°C
Aplicação
Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.
Informações legais
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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