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14479

Sigma-Aldrich

Phalloidin-Atto 490LS

suitable for fluorescence

Sinônimo(s):

Atto 490LS-Phalloidin

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About This Item

Código UNSPSC:
12352108
NACRES:
NA.32

Ensaio

≥75.0% (HPLC)

peso molecular

Mw 1466 g/mol

fabricante/nome comercial

ATTO-TEC GmbH

λ

in acetonitrile: water (1:1)

Absorção UV

λ: 495-501 nm Amax

adequação

suitable for fluorescence

temperatura de armazenamento

−20°C

Descrição geral

Atto 490LS is a new fluorescent label featuring an extraordinary large Stokes-Shift of 165 nm. Thus the emission spectrum is almost completely separated from its absorption spectrum, making the dye highly suitable for multiplexing experiments, in particular in combination with Atto 488 and Atto 514. Atto 490LS is very hydrophillic and shows excellent water solubility. The dye exhibits a relatively high fluorescence quantum yield, which is only slightly reduced after conjugation to biomolecules, e.g. proteins, even at high degrees of labeling (DOL). Atto 490LS is an anionic dye. After conjugation to a substrate the dye carries a net electrical charge of -1.
Phalloidin, a bicyclic heptapeptide, is a very strong binding reagent to actin. Fluorescent labeled phalloidin has become a useful tool to investigate the distribution of F-actin within the cytoskeleton of cells by fluorescence microscopy.

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Informações legais

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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