IPTG-RO
Roche
β-D-1-tiogalactopiranosídeo isopropílico
mol wt 238.3, pkg of 1 g (10724815001), pkg of 5 g (11411446001)
Sinônimo(s):
β-D-tiogalactosídeo isopropílico, IPTG
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About This Item
Fórmula empírica (Notação de Hill):
C9H18O5S
Número CAS:
Peso molecular:
238.30
Beilstein:
4631
Número MDL:
Código UNSPSC:
12352200
ID de substância PubChem:
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Formulário
powder
peso molecular
238.3
embalagem
pkg of 1 g (10724815001)
pkg of 5 g (11411446001)
fabricante/nome comercial
Roche
Condições de expedição
wet ice
temperatura de armazenamento
2-8°C
cadeia de caracteres SMILES
CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O
InChI
1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
chave InChI
BPHPUYQFMNQIOC-NXRLNHOXSA-N
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Descrição geral
Isopropyl-β-D-thiogalactoside (IPTG) is a chemical analog of galactose that cannot be cleaved by β-galactosidase. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Aplicação
O IPTG é comumente usado nos procedimentos de clonagem que requerem indução da atividade de β-galactosidase. Ele é usado em conjunto com X-Gal ou Bluo-Gal na seleção azul-branca de colônias bacterianas recombinantes que induzem a expressão do operon lac em Escherichia coli. O IPTG age ligando-se ao repressor lacI e alterando sua conformação, o que impede a repressão do gene lacZ que codifica a β-galactosidase.
Isopropyl-β-D-thiogalactoside (IPTG) is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. It has been used for the expression of recombinant genes in E. coli.
Outras notas
Análogo de galactose não metabolizável.
For life science research only. Not for use in diagnostic procedures.
The amount of labeled DNA depends on:
- amount of template DNA
- purity of template DNA
- conformation of template DNA
- average size of labeled fragment
Especificações
Assay Time: 50minutes
Specific Activity: The described standard assay will results in a specific activity of 1.8 x 109 dpm/μg, corresponding to 65% incorporation with different substrate DNAs in 30minutes. When varying the ratio of template DNA to labeled dNTP, similar incorporation rates, but different levels of specific activity of the labeled probe are obtained.
Specific Activity: The described standard assay will results in a specific activity of 1.8 x 109 dpm/μg, corresponding to 65% incorporation with different substrate DNAs in 30minutes. When varying the ratio of template DNA to labeled dNTP, similar incorporation rates, but different levels of specific activity of the labeled probe are obtained.
Nota de preparo
Sample Material
10 ng to 3μg template DNA, linearized and at least 100 to 200bp long
10 ng to 3μg template DNA, linearized and at least 100 to 200bp long
Working concentration: Final concentration should be 0.2 mM.
Storage conditions (working solution): Stock solutions of the product in water (e.g., 1 M IPTG) stored at -15 to -25 °C, are stable up to six months.
Storage conditions (working solution): Stock solutions of the product in water (e.g., 1 M IPTG) stored at -15 to -25 °C, are stable up to six months.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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The role of the Escherichia coli lacY gene product (the lactose permease) in the induction of isopropyl-beta-D-thiogalactopyranoside (IPTG) inducible promoters was studied in E. coli and P. fluorescens. This was done by comparing strains containing a lacIPOZYA chromosomal insert with
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