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11388908910

Roche

Biotin-16-UTP

pkg of 25 μL (250 nmol; 10mM)

Sinônimo(s):

Biotin-16-UTP, biotin

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About This Item

Código UNSPSC:
41116100

Ensaio

97.7% (HPLC)

Nível de qualidade

100

forma

solution

peso molecular

Mr 987.5 (biotin-16-UTP-Li4)

embalagem

pkg of 25 μL (250 nmol; 10mM)

fabricante/nome comercial

Roche

cor

colorless

solubilidade

water: miscible

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Descrição geral

For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.

Aplicação

Biotin-labeled RNA can be used as a hybridization probe for:
  • Northern blots
  • Southern blots
  • Plaque or colony lifts
  • RNase protection experiments
  • In situ hybridization
  • Microarray hybridization
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.

Qualidade

Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).

forma física

Biotin-16-UTP, tetralithium salt, 10mM solution

Outras notas

For life science research only. Not for use in diagnostic procedures.

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

does not flash

Ponto de fulgor (°C)

does not flash

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Homoharringtonine, a plant alkaloid, has been reported to suppress protein synthesis and has been approved by the US Food and Drug Administration for the treatment of chronic myeloid leukemia. Here we show that in acute myeloid leukemia (AML), homoharringtonine potently
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Methamphetamine (METH) is an illicit toxic psychostimulant which is widely abused. Its toxic effects depend on the release of excessive levels of dopamine (DA) that activates striatal DA receptors. Inhibition of DA-mediated neurotransmission by the DA D1 receptor antagonist, SCH23390
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Vascular smooth muscle cell (VSMC) proliferation causes intimal thickening in atherosclerosis and restenosis. Previously, we demonstrated that Wnt/β-catenin signaling upregulates VSMC proliferation in vitro. We examined this pathway in vivo and investigated the involvement of specific Wnt proteins in VSMC

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