03708152001
Roche
Streptavidin Mutein Matrix
suspension, pkg of 5 mL
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12352200
NACRES:
NA.21
Produtos recomendados
forma
suspension
embalagem
pkg of 5 mL
fabricante/nome comercial
Roche
Parâmetros
4-25 °C optimum reaction temp.
Condições de expedição
wet ice
Descrição geral
Contents
Settled resin of 50% suspension Streptavidin Mutein Matrix.
Settled resin of 50% suspension Streptavidin Mutein Matrix.
Streptavidin mutein, immobilized.
The binding of biotin to avidin/streptavidin is the strongest known noncovalent interaction of biological molecules [(Kd 0.6 x 10-15 M (avidin), 4 x 10-14 M (streptavidin)]. Strong binding provides a great advantage for certain applications of the derived biotin-avidin/streptavidin technology, such as surface immobilization, blotting technologies, immunoassays, cross-linking studies, and histochemistry. However, the use of immobilized avidin/streptavidin for the purification of biotinylated proteins is limited. In this instance, the extreme affinity of the binding partners creates a drawback of enabling elution only under very harsh conditions that usually destroy the functionality of the protein of interest.
Recombinant streptavidin technology was used to screen for a mutant streptavidin with reduced binding affinity toward biotin. By substituting three amino acids, a mutein was obtained with a biotin dissociation constant of 1.3 x 10-7 M.
The Streptavidin Mutein Matrix provides this mutein immobilized onto cross-linked agarose beads in a highly stable and regenerable form, which allows purification of biotinylated proteins that results in excellent purity and recovery.
Recombinant streptavidin technology was used to screen for a mutant streptavidin with reduced binding affinity toward biotin. By substituting three amino acids, a mutein was obtained with a biotin dissociation constant of 1.3 x 10-7 M.
The Streptavidin Mutein Matrix provides this mutein immobilized onto cross-linked agarose beads in a highly stable and regenerable form, which allows purification of biotinylated proteins that results in excellent purity and recovery.
Aplicação
- Streptavidin Mutein Matrix: Is highly suitable for purification of biotinylated proteins
- Has been tested for use with in vitro expressed biotinylated proteins, using the RTS AviTag Biotinylation Kits
- Is convenient for purification of chemically biotinylated proteins
- It was also used in single-step affinity chromatography and affinity chromatography.
Características e benefícios
- Mild purification conditions: Low biotin dissociation constant allows elution of biotinylated proteins under gentle conditions.
- Stable: No significant loss of binding capacity upon storage. The low dissociation constant of the streptavidin mutein is obtained by genetic engineering and not by dissociation of avidin- or streptavidin tetramers.
- Economic: High binding capacity and multiple reuse allows economic handling of the matrix.
Armazenamento e estabilidade
Store at 2 to 8 °C. (Do not freeze!)
Outras notas
For life science research only. Not for use in diagnostic procedures.
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
does not flash
Ponto de fulgor (°C)
does not flash
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
Nancy G Nossal et al.
The Journal of biological chemistry, 282(2), 1098-1108 (2006-11-16)
Our previous electron microscopy of DNA replicated by the bacteriophage T4 proteins showed a single complex at the fork, thought to contain the leading and lagging strand proteins, as well as the protein-covered single-stranded DNA on the lagging strand folded
Dalia Juzumiene et al.
Nuclear receptor signaling, 3, e001-e001 (2006-04-11)
The full-length human androgen receptor with an N-terminal biotin acceptor peptide tag was overexpressed in Spodoptera frugiperda cells in the presence of 1 microM dihydrotestosterone. Site-specific biotinylation of BAP was achieved in vivo by co-expression of E. coli biotin holoenzyme
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica