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Key Documents

11204530001

Roche

ABTS Buffer

solution, pkg of 125 mL

Sinônimo(s):

buffer

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About This Item

Código UNSPSC:
12352204

forma

solution

Nível de qualidade

embalagem

pkg of 125 mL

fabricante/nome comercial

Roche

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Descrição geral

2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts as a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS is a highly sensitive, specific and reproducible technique.

Aplicação

ABTS Buffer is a solvent for ABTS. ABTS solution is the ideal substrate solution for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme. Because of the high sensitivity of the reaction, autoreaders will often display "over" after several minutes.

Nota de preparo

Working concentration: Flow cytometry: 0.5 μg/100 μl (106 cells); Immunohistocytochemistry: 50 μg/ml
Working concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.
Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of ABTS buffer. This solution has a light-green color. In microtiter plates, the solution is colorless A405 nm/1 cm should be <0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações legais

ABTS is a trademark of Roche

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

No data available

Ponto de fulgor (°C)

No data available


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C Lindenthal et al.
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H Matsuda et al.
The Japanese journal of experimental medicine, 54(3), 131-138 (1984-06-01)
A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, 2,2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as a substrate for horseradish peroxidase (HRP) conjugate was studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for
Bruce S Sachais et al.
Blood, 120(5), 1137-1142 (2012-05-12)
Rapid laboratory assessment of heparin-induced thrombocytopenia (HIT) is important for disease recognition and management. The utility of contemporary immunoassays to detect antiplatelet factor 4 (PF4)/heparin antibodies is hindered by detection of antibodies unassociated with disease. To begin to distinguish properties

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