11088939001

Tumor Necrosis Factor-α, human (hTNF-α)

recombinant (yeast)

• Sinônimo(s): hTNF, human necrosis factor
• Código UNSPSC: 12352207

Propriedades

• fonte biológica: human
• Nível de qualidade: 100
• recombinante: expressed in yeast
• Ensaio: ≥95% (SDS-PAGE)
• Formulário: solution
• potência: ≤0.01 ng/mL EC₅₀
• atividade específica: >1*10^8 U/mg
• peso molecular: 17,000 Da
• embalagem: pkg of 1,000,000 U (10 μg, 1ml)
• fabricante/nome comercial: Roche
• condição de armazenamento: avoid repeated freeze/thaw cycles
• Impurezas: <0.1 EU/μgtested (LAL test)
• nº de adesão UniProt: Q9UBM5
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... TNF(7124)

Descrição

Descrição geral

>1 x 10^⊥8 U/mg, <0.01 ng/ml (hTNF-a, NIBSC, interim standard, 87/650), at least the same specific activity (EC50) compared to the indicated standard is guaranteed.

Especificidade

Human hTNF-α is effective on human and mouse cells.

Species specificity: human, mouse

Aplicação

Tumor Necrosis Factor-α (hTNF-α)

• Shows cytolytic and/or cytostatic activity on a variety of transformed cell lines.
• Has activating and growth stimulating activities on a variety of normal cells.
• Has antiviral activity on many cell types in vitro.
• Can cause tumor necrosis of certain tumors in vivo.

Ações bioquímicas/fisiológicas

Receptor p55 and p75, belonging to the TNFR gene family, mediate the biological activity of TNF-a, and are co-expressed on the surface of various cell types. The pleiotropic action of TNF-a is initiated by binding to either of the two receptors.

Qualidade

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)

Sequência

Chain Length 157 AA

One polypeptide chain (157 amino acids), identical to natural hTNF-α.

Definição da unidade

EC₅₀ definition: The amount of hTNF-α that is required to mediate half-maximal cytotoxicity (MTT cleavage) with WEHI 164 cells in the presence of actinomycin D (1 unit equals ≤0.01 ng/ml).

forma física

Solution, filtered through 0.2 μm pore size membrane.

Nota de preparo

Working concentration: 1 ng/ml
For complete cell lysis of sensitive cell lines about 1 ng/ml is recommended.
Working solution: Dilute the concentrated hTNF-α solution (10 μg/ml) with PBS or culture medium containing BSA (or HSA), 1 mg/ml (0.1%) or serum 1 to 10%.
Storage conditions (working solution): -15 to -25 °C
It is recommended to store the solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Outras notas

For life science research only. Not for use in diagnostic procedures. 1 EU corresponds to 0.1 ng

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash