NE1013

Anti-PGP9.5 (176-191) Rabbit pAb

liquid, Calbiochem^®

• Sinônimo(s): Anti-Neuron Cytoplasmic Protein 9.5, Anti-Ubiquitin Thiolesterase L1, Anti-UCHL1
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: serum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• forma: liquid
• contém: ≤0.1% sodium azide as preservative
• reatividade de espécies: porcine, human, rat, mouse
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles
• Isotipo: IgG
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... UCHL1(7345)

Descrição

Descrição geral

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~26 kDa PGP9.5 protein.

Recognizes the ~26 kDa PGP9.5 protein in mouse dorsal root nerve fibers.

This Anti-PGP9.5 (176-191) Rabbit pAb is validated for use in Immunoblotting, Frozen Sections for the detection of PGP9.5 (176-191).

Imunogênio

a synthetic peptide (ASSEDTLLKDAAKVCR) corresponding to amino acids 176-191 of PGP9

Aplicação

Immunoblotting (1:1000)
Frozen Sections (1:2000, fluorescence)

Advertência

Toxicity: Standard Handling (A)

forma física

Undiluted serum.

Reconstituição

Following initial thaw, aliquot and freeze (-20°C).

Nota de análise

Positive Control
Mouse dorsal root nerve fibers

Outras notas

Caballero, O.L., et al. 2002. Oncogene21, 3003.
Tezel, E., et al. 2000. Clin. Cancer Res.6, 4764.

PGP9.5 is also overexpressed in some cancers.



Immunofluorescence protocol

Care should be taken so that the incubation solutions do not evaporate. It is recommended that incubations be performed in a humidified chamber to prevent evaporation. Washing steps should be done in large volumes of phosphate buffered saline (PBS).

1. Warm slides to room temperature.

2. Re-hydrate with PBS for 10-15 min.

3. Remove PBS and incubate in blocking buffer (PBS containing 3% normal donkey serum, 1% bovine serum albumin, 0.3% Triton^™ X-100 detergent, and 0.01% NaN₃, pH 7.45) for 1 h at room temperature.

4. Dilute primary antibody in blocking buffer. Store diluted antibody at 4°C until use.

5. Remove blocking buffer and add diluted primary antibody.

6. Incubate with primary antibody at 4°C for 18-48 h.

7. Remove primary antibody and wash three times for 10 min with PBS.

8. Dilute secondary antibody in blocking buffer to appropriate dilution (follow manufacture′s recommendation). Store at 4°C until use.

9. Incubate slides in diluted secondary antibody solution for one hour at room temperature.

10. Remove secondary antibody and wash three times for 10 min with PBS.

11. Coverslip slides with appropriate mounting media.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1