MABT523
Anti-beta-Actin Antibody, clone RM112
clone RM112, from rabbit
Sinônimo(s):
Actin, cytoplasmic 1, Beta-actin, beta-Actin
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
purified antibody
tipo de produto de anticorpo
primary antibodies
clone
RM112, monoclonal
reatividade de espécies
human
técnica(s)
immunocytochemistry: suitable
western blot: suitable
Isotipo
IgG
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... ACTB(60)
Descrição geral
Actin is a 43 kDa highly conserved structural protein. There are three main actin isotypes (alpha, beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. Beta actin is one of the two nonmuscle cytoskeletal actins and is relatively stable and appears to remain at normal levels regardless of experimental treatment. It is generally used as an internal control for experiments.
Imunogênio
A peptide corresponding to the N-terminus of beta-Actin.
Aplicação
Immunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected beta-Actin in A431 cells.
This Anti-beta-Actin Antibody, clone RM112 is validated for use in Western Blotting, Immunocytochemistry for the detection of beta-Actin.
Qualidade
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected beta-Actin in A431 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected beta-Actin in A431 cell lysate.
Descrição-alvo
~45 kDa observed. Uncharacterized bands may be observed in some lysate(s).
forma física
Format: Purified
Outras notas
Concentration: Please refer to lot specific datasheet.
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Preços
Classe de risco de água (WGK)
WGK 2
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