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Key Documents

MABS2291

Sigma-Aldrich

Anti-DPP4/CD26 Antibody, clone KU44.13A

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

KU44.13A, monoclonal

peso molecular

calculated mol wt 88.28 kDa

purificado por

using protein G

reatividade de espécies

human

embalagem

antibody small pack of 100 μL

técnica(s)

ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable

Isotipo

IgG2κ

sequência de epítopo

Extracellular domain

nº de adesão de ID de proteína

nº de adesão UniProt

Condições de expedição

2-8°C

modificação pós-traducional do alvo

unmodified

Descrição geral

Dipeptidyl peptidase 4 (UniProt: P27487; also known as EC:3.4.14.5, ADABP, Adenosine deaminase complexing protein 2, ADCP-2, Dipeptidyl peptidase IV, DPP IV, T-cell activation antigen CD26, TP103, CD26) is encoded by the DPP4 (also known as CD26, ADCP2) gene (Gene ID: 1803) in human. DPP4 is a single-pass type II membrane glycoprotein that is ubiquitously expressed on the surface of a variety of cells. It is expressed in endothelial cells, fibroblasts, and lymphocytes and on the apical surfaces of epithelial and acinar cells. It serves as a serine exopeptidase that cleaves X-proline or X-alanine dipeptides from the N-terminus of polypeptides. A soluble form of DPP4 is also present (aa 39-766) that is derived from the membrane by proteolytic processing. DPP4 expression is substantially dysregulated in a variety of disease states including inflammation, cancer, obesity, and diabetes and its inhibition by the gliptin family of drugs has gained importance for the therapy of type 2 diabetes. DPP4 is also involved in the costimulatory signal that is essential for T-cell receptor (TCR)-mediated T-cell activation. Its levels are up-regulated by IL-12 on activated T-cells and down-regulated by TNF. DPP4 also plays a role in tumor development and its effect depends on the tumor type and its microenvironment. It has a tumor suppressor effect in several cancers including melanoma, ovarian cancer, non-small cell lung cancer, prostate cancer, endometrial cancer, and neuroblastoma. Higher levels of DPP4 have been observed in pancreatic cancer and knockdown of DPP4 expression inhibits cell growth, migration, and invasion. (Ref.: Arias-Pinilla, GA., et al. (2020). Sci. Rep. 10; 537; Röhrborn, D., et al. (2015). Front. Immunol. 6; 386).

Especificidade

Clone KU44.13A is a mouse monoclonal antibody that detects Dipeptidyl peptidase 4 (DPP4)/CD26.

Imunogênio

CFPAC-1 human pancreatic cancer cells and SP2 myeloma cells.

Aplicação

Quality Control Testing

Evaluated by Flow Cytometry in HPAF-II cells.

Flow Cytometry Analysis: 1 μg of this antibody detected DPP4/CD26 in one million HPAF-II cells.

Tested Applications

Immunofluorescence Analysis: A representative lot detected DPP4/CD26 in Immunofluorescence applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep. 10(1):537).

Immunoprecipitation Analysis: A representative lot immunoprecipitated DPP4/CD26 in Immunoprecipitation applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep.10(1):537).

ELISA Analysis: A representative lot detected DPP4/CD26 in ELISA applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep. 10(1):537).

Flow Cytometry Analysis: A representative lot detected DPP4/CD26 in Flow Cytometry applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep. 10(1):537).

Immunohistochemistry Applications: A representative lot detected DPP4/CD26 in Immunohistochemistry applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep. 10(1):537).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
This Anti-DPP4/CD26, clone KU44.13A, Cat. No. MABS2291, is a mouse monoclonal antibody that detects DPP4/CD26 and is tested for use in ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Immunoprecipitation.

forma física

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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