Pular para o conteúdo
Merck
Todas as fotos(1)

Documentos Principais

MABF2812

Sigma-Aldrich

Anti-CD137 Antibody, clone LOB12.0

Sinônimo(s):

4-1BB ligand receptor, T-cell antigen 4-1BB, Tumor necrosis factor receptor superfamily member 9

Faça loginpara ver os preços organizacionais e de contrato


About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

rat

Nível de qualidade

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

LOB12.0, monoclonal

peso molecular

calculated mol wt 27.6 kDa
observed mol wt ~N/A kDa

purificado por

using protein G

reatividade de espécies

mouse

embalagem

antibody small pack of 100

técnica(s)

competitive inhibition ELISA: suitable
flow cytometry: suitable
inhibition assay: suitable
surface plasmon resonance (SPR): suitable

Isotipo

IgG2aκ

sequência de epítopo

Unknown

nº de adesão de ID de proteína

nº de adesão UniProt

temperatura de armazenamento

-10 to -25°C

Informações sobre genes

mouse ... Tnfrsf9(21942)

Especificidade

Clone LOB12.0 is a rat monoclonal antibody that detects CD137 (Tnfrsf9).

Imunogênio

Mouse CD137 human Fc fusion protein

Aplicação

Quality Control Testing

Isotype testing: Identity confirmation by Isotyping test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be rat IgG2a .

Tested Applications

Surface plasmon resonance: A representative lot detected CD137 in Surface plasmon resonance applications (Buchan, S.L., et al. (2018). Immunity. 49(5):958-970.e.7).

Flow Cytometry Analysis: 10 µg from a representative lot detected CD137 in Jurkat cells expressing mouse CD137.

Agonist Activity: A representative lot of this antibody co-stimulated CD8+ T cells and potentiated anti-tumor immunity in mice.(Taraban, V.Y., et al. (2002). Eur J Immunol. 32(12); 3617-3627 ).

Competition Assay: A representative lot of this antibody competed for binding to 4-1BB on effector T cells. (Buchan, S.L., et al. (2018). Immunity. 49(5):958-970.e.7).

Flow Cytometry Analysis: A representative lot detected CD137 in Flow Cytometry applications (Buchan, S.L., et al. (2018). Immunity. 49(5):958-970.e.7).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrição-alvo

Tumor necrosis factor receptor superfamily member 9 (UniProt: P20334; also known as 4-1BB ligand receptor, T-cell antigen 4-1BB, CD137) is encoded by the Tnfrsf9 (also known as Cd137, IIa, Ly63) gene (Gene ID: 21942) in murine species. CD137 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 24-187), a transmembrane domain (aa 187-208), and a cytoplasmic domain (aa 209-256). It is a member of the TNFR superfamily that serves as an activation-induced costimulatory molecule. Its expression is observed on activated T cells, NK cells, dendritic cells, eosinophils, mast cells, endothelial cells, and some tumor cells. It is rapidly expressed on activated CD8+ T cells and at lower levels on activated CD4+ T cells. Induction of CD137 expression on CD8+ T cells is reported to be highly sensitive to low levels of TCR stimulation. Engagement of CD137 on T cells stimulates their proliferation independently of CD28 and promotes IL-2 and IFN- secretion by Th effector cells and CTLs, respectively. Expression of CD137 is shown to negatively correlate with lymphocyte proliferation and positively with the degree of activation-induced cell death caused by mitogen overstimulation. Treg cells that infiltrate tumors are reported to express higher levels of CD137 and administration of clone LOB12.0 with agonistic function promotes T cell agonism to promote tumor rejection. (Ref.: Buchan, SL., et al. (2018). Immunity 49(5), 958-970; Taraban, VY., et al. (2002). Eur. J. Immunol. 32(12); 3617-3627).

forma física

Purified rat monoclonal antibody IgG2a in PBS without preservatives.

Reconstituição

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Não está encontrando o produto certo?  

Experimente o nosso Ferramenta de seleção de produtos.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica