MABE915

Anti-Mi-2β Antibody, clone 2G8

clone 2G8, from mouse

• Sinônimo(s): Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, Mi-2b
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clone: 2G8, monoclonal
• application: ChIP; WB
• reatividade de espécies: human
• técnica(s): ChIP: suitable (ChIP-seq); western blot: suitable
• citations: 1

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 2G8, monoclonal
• reatividade de espécies: human
• técnica(s): ChIP: suitable (ChIP-seq); western blot: suitable
• Isotipo: IgG2bκ
• nº de adesão NCBI: NP_001264
• nº de adesão UniProt: Q14839
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... CHD4(1108)

Descrição

Descrição geral

Chromodomain-helicase-DNA-binding protein 4 (CHD-4), often referred to as Mi-2β, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. Mi-2β has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of Mi-2β.

Aplicação

Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody used as part of an antibody cocktail of consisting of Anti-Mi-2β clones 2G8, 16G4 and 17H11 (cat. no. MABE915, MABE914, and MABE920 respectively) was reported to work in ChIP (see Zhang, J., et al 2012).

ChIP-seq: A representative lot of this antibody used as part of an antibody cocktail of consisting of Anti-Mi-2β clones 2G8, 16G4 and 17H11 (cat. no. MABE915, MABE914, and MABE920 respectively) was reported to work in ChIP-seq (see Zhang, J., et al 2012).

This Anti-Mi-2β Antibody, clone 2G8 is validated for use in Western Blotting and ChIP-seq and Culture Confirmation for the detection of Mi-2β.

Qualidade

Evaluated by Western Blotting in a Jurkat cell lysate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Mi-2β in 10 µg of a Jurkat cell lysate.

Descrição-alvo

~ 220 kDa observed

forma física

Format: Purified

Outras notas

Concentration: Please refer to lot specific datasheet.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable