MABC28

Anti-RIP3 Antibody, clone 6E6.2

clone 6E6.2, from mouse

• Sinônimo(s): Receptor-interacting serine/threonine-protein kinase 3, RIP-like protein kinase 3, Receptor-interacting protein 3, RIP-3
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 6E6.2, monoclonal
• reatividade de espécies: human, rat
• técnica(s): immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG2aκ
• nº de adesão NCBI: NP_006862
• nº de adesão UniProt: Q9Y572
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... RIPK3(11035)

Descrição

Descrição geral

Receptor-interacting protein 3 (RIP3) contains a N-terminal kinase domain and interacts with RIP. The binding of RIP to RIP3 causes the RIP3/RIP complex to be shuttled into the tumor necrosis factor receptor-1 signaling cascade. RIP3 is known to be a very effective apoptosis inducer. Two novel splice variants of RIP3 have been discovered, RIP3 beta and RIP3 gamma, that have shown to decrease apoptosis related to full length RIP3. RIP3 gamma has been shown to be significantly up-regulated in colon and lung cancers and may play a role in tumorigenesis.

Especificidade

This antibody recognizes RIP3 at the N-terminus.

Imunogênio

Epitope: N-terminus

GST-tagged recombinant protein corresponding to human RIP3 at the N-terminus.

Aplicação

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-RIP3 Antibody, clone 6E6.2 is validated for use in WB, IH for the detection of RIP3.

Western Blot Analysis: A 1:1,000 dilution from a representative lot detected RIP3 in 10 µg of human placenta tissue lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected RIP3 in rat pancreas tissue.

Qualidade

Evaluated by Western Blot in human pancreas tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected RIP3 in 10 µg of human pancreas tissue lysate.

Descrição-alvo

~56 kDa (isoform 1) and ~27 kDa (isoform 2) observed.
Isoform 3 (~25 kDa) may be observed in some lysates. An uncharacterized band may be observed at ~16 kDa in some lysates.

forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Human pancreas tissue lysate

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable