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MABC004

Sigma-Aldrich

Mouse IgG2a Negative Control, clone GC270

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.42

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified antibody

clone

GC270, monoclonal

fabricante/nome comercial

Chemicon®

concentração

0.5 mg/mL

técnica(s)

flow cytometry: suitable
immunofluorescence: suitable

Isotipo

IgG2a

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Especificidade

This antibody reacts with the outer membrane protein of some strains of Neisseria gonorrhoeae. No reaction with human cell surface or plasma components has been observed. However, some binding to Fc receptors may occur.

Imunogênio

Protein I preparation from Neisseria gonorrhoeae.

Aplicação

Mouse IgG2a is a monoclonal antibody intended for use as a negative control in immunofluorescence, flow cytometry, and immunoperoxidase staining on frozen and paraffin tissue sections. Its use enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.

SUGGESTED USAGE

The control antibody should be diluted to the same concentration as the test antibody, and equivalent volumes used.
Research Category
Secondary & Control Antibodies

Epitope Tags & General Use
Research Sub Category
Isotype Control Antibodies

Ligação

Replaces: CBL601

forma física

Format: Purified
Purified from mouse ascitic fluid. The antibody is supplied in 0.5ml 25mM Tris-HCl, 0.4M NaCl, pH 8.0, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis, specific immunofluorescence assay and flow cytometry.

Armazenamento e estabilidade

Store at 2 to 8°C, for up to 18 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

not combustible

Ponto de fulgor (°C)

not combustible


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International journal of toxicology, 36(5), 386-394 (2017-08-19)
Immunohistochemical staining for the lysosome-associated membrane protein 2 (LAMP-2) has been proposed previously as an alternative to electron microscopy to identify hepatic phospholipidosis. This study used LAMP-2 immunohistochemistry (IHC) to diagnose phospholipidosis in rats exhibiting renal tubular injury. Rats were
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Memorias do Instituto Oswaldo Cruz, 112(6), 458-468 (2017-06-08)
Dengue fever may present hemorrhages and cavitary effusions as result of exacerbated immune responses. We investigated hydro-alcoholic extracts from leaves (UGL) and bark (UGB) of the medicinal species Uncaria guinanensis with respect to antiviral effects in Dengue virus (DENV) infection
Adenovirus E4orf4 protein-induced death of p53-/- H1299 human cancer cells follows a G1 arrest of both tetraploid and diploid cells due to a failure to initiate DNA synthesis.
Cabon, L; Sriskandarajah, N; Mui, MZ; Teodoro, JG; Blanchette, P; Branton, PE
Journal of virology null

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