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Documentos Principais

MAB5446

Sigma-Aldrich

Anti-Neurofilament NF-H Antibody, tail domain (Phosphate dependent), clone RMO-24

ascites fluid, clone RMO-24, Chemicon®

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

ascites fluid

tipo de produto de anticorpo

primary antibodies

clone

RMO-24, monoclonal

reatividade de espécies

human, rat

fabricante/nome comercial

Chemicon®

técnica(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG1κ

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

dry ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... NEFH(4744)
rat ... Nefh(24587)

Categorias relacionadas

Especificidade

Reacts with Neurofilament Heavy (NF-H), tail domain (phosphate dependent). Dephosphorylation of the NF-H will results in diminished immunoreactivity.

Imunogênio

Epitope: tail domain (Phosphate dependent)
Rat Neurofilaments

Aplicação

Anti-Neurofilament NF-H Antibody, tail domain (Phosphate dependent), clone RMO-24 detects level of Neurofilament NF-H & has been published & validated for use in ELISA, IP, WB, IH.
Research Category
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers
Western blot. Reacts with the 200 kD NF-H from human.

Immunohistochemistry. The antibody has been used successfully on Bouin′s and alcohol-fixed paraffin-embedded or frozen tissue sections. Formalin-fixed paraffin-embedded tissue sections require pretreatment with trypsin.

Immunoprecipitation

Optimal working dilutions must be determined by end user.

forma física

Ascites fluid. Liquid.

Armazenamento e estabilidade

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Nota de análise

Control
Positive Control: Brain

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Hirofumi Tokuoka et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 26(45), 11606-11614 (2006-11-10)
The mechanism by which synaptic vesicles (SVs) are recruited to the release site is poorly understood. One candidate mechanism for trafficking of SVs is the myosin-actin motor system. Myosin activity is modulated by myosin light chain kinase (MLCK), which in
V M Lee et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 7(11), 3474-3488 (1987-11-01)
A new panel of greater than 300 monoclonal antibodies (mAbs) was prepared to the high, middle, and low Mr rat neurofilament (NF) subunits (NF-H, NF-M and NF-L, respectively). NF proteins were purified both from native, i.e., phosphorylated rat NFs and

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