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MAB5428

Sigma-Aldrich

Anti-Retinal Pigment Epithelium 65 Antibody

clone 401.8B11.3D9, Chemicon®, from mouse

Sinônimo(s):

RPE65

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Preço e disponibilidade não estão disponíveis no momento.

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

401.8B11.3D9, monoclonal

reatividade de espécies

bovine, human, Xenopus, mouse

embalagem

antibody small pack of 25 μg

fabricante/nome comercial

Chemicon®

técnica(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG

Especificidade

Reacts with Retinal Pigment Epithelium 65 (RPE65). On bovine RPE membranes the antibody recognizes a protein with a molecular weight of ~65 kDa.

Imunogênio

Bovine RPE microsomal membranes.

Aplicação

Anti-Retinal Pigment Epithelium 65 Antibody detects level of Retinal Pigment Epithelium 65 & has been published & validated for use in ELISA, IH, IP & WB.
Research Category
Neuroscience
Research Sub Category
Sensory & PNS
Western blot: 1:5,000-1:20,000 on bovine RPE membranes using ECL. Suggested dilution buffer is TBS containing 10% calf serum, 0.25% T-20, 1M D-glucose with 10% glycerol. Suggested blocking buffer is TBS containing 2% BSA and 0.5% Tween 20. Preferred gel percentage is 10%.

Immunohistochemistry on frozen tissue sections: 1:250-1:500

Immunoprecipitation: 20 μg of antibody in a reaction volume of 500 μL.

Immunoaffinity purification

ELISA

Optimal working dilutions must be determined by end user.

Descrição-alvo

~ 65 kDa

forma física

Format: Purified
Protein A purified
Purified immunoglobulin. Liquid in PBS. Contains no preservative.

Armazenamento e estabilidade

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Nota de análise

Control
Eye

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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