MAB3480
Anti-CFTR Antibody, a.a. 1370-1380, clone M3A7
clone M3A7, Chemicon®, from mouse
Sinônimo(s):
CFTR
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
M3A7, monoclonal
reatividade de espécies
human
não deve reagir com
mouse, shark, rat
fabricante/nome comercial
Chemicon®
técnica(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG1
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... CFTR(1080)
Descrição geral
CFTR (Cystic Fibrosis Transmembrane Regulator) is an integral membrane protein shown to be involved in the transport of chloride ions. It is found on the surface of the epithelial cells lining the lungs and other organs. CFTR defects are the cause of cystic fibrosis.
Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508) which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86% and 100% reductions in S.typhi intestinal submucosal uptake.
Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508) which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86% and 100% reductions in S.typhi intestinal submucosal uptake.
Especificidade
Recognizes Human cystic fibrosis transmembrane conductance regulator (CFTR). Recognizes an epitope at the C-terminal end of the second nucleotide binding in the region of residues 1370 to 1380.
Imunogênio
Epitope: a.a. 1370-1380
Recombinant protein encoding NBF2 domain of human CFTR
Aplicação
Anti-Cystic Fibrosis Transmembrane Conductance Regulator Antibody, a.a. 1370-1380, clone M3A7 detects level of Cystic Fibrosis Transmembrane Conductance Regulator & has been published & validated for use in IC, IP & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Western blot: 1μg/mL, do not boil, recognizes 170kDa CTFR protein.
Immunoprecipitation
Immunofluorescence
T84 adenocarcinoma cells are a good positive control
Immunohistochemistry: 1-50μg/mL on acetone fixed frozen sections. Does not work on paraffin embedded tissue.
Optimal working dilutions must be determined by the end user.
Immunoprecipitation
Immunofluorescence
T84 adenocarcinoma cells are a good positive control
Immunohistochemistry: 1-50μg/mL on acetone fixed frozen sections. Does not work on paraffin embedded tissue.
Optimal working dilutions must be determined by the end user.
Descrição-alvo
170 kDa
forma física
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified
Armazenamento e estabilidade
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Nota de análise
Control
T84 whole cell extract
T84 whole cell extract
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Descrição
Preços
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
C terminus of nucleotide binding domain 1 contains critical features for cystic fibrosis transmembrane conductance regulator trafficking and activation.
Billet, A; Melin, P; Jollivet, M; Mornon, JP; Callebaut, I; Becq, F
The Journal of Biological Chemistry null
Establishment and characterization of a novel polarized MDCK epithelial cellular model for CFTR studies
Mendes, Filipa, et al
Cellular Physiology and Biochemistry, 16, 281-290 (2005)
Paul D W Eckford et al.
The Journal of biological chemistry, 287(44), 36639-36649 (2012-09-04)
The cystic fibrosis transmembrane conductance regulator (CFTR) acts as a channel on the apical membrane of epithelia. Disease-causing mutations in the cystic fibrosis gene can lead to CFTR protein misfolding as in the case of the F508del mutation and/or channel
Renaud Robert et al.
Molecular pharmacology, 73(2), 478-489 (2007-11-03)
The F508del mutation impairs trafficking of the cystic fibrosis transmembrane conductance regulator (CFTR) to the plasma membrane and results in a partially functional chloride channel that is retained in the endoplasmic reticulum and degraded. We recently used a novel high-throughput
Unusually common cystic fibrosis mutation in Portugal encodes a misprocessed protein.
Mendes, Filipa, et al.
Biochemical and biophysical research communications, 311, 665-671 (2003)
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