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Key Documents

MAB1919

Sigma-Aldrich

Anti-Fibrillin Antibody, clone 11C1.3

clone 11C1.3, Chemicon®, from mouse

Sinônimo(s):

Anti-ACMICD, Anti-FBN, Anti-GPHYSD2, Anti-MASS, Anti-MFLS, Anti-MFS1, Anti-OCTD, Anti-SGS, Anti-SSKS, Anti-WMS, Anti-WMS2

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

11C1.3, monoclonal

reatividade de espécies

bovine, pig, human

fabricante/nome comercial

Chemicon®

técnica(s)

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG1κ

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

dry ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... FBN1(2200)

Especificidade

Bovine zonular fibrils. Reacts well with human elastin microfibrils and can be used to investigate the presence and organization of these fibrils either in Marfan patients or in fibroblasts obtained from these patients.

Imunogênio

Bovine zonular fibrils

Aplicação

Anti-Fibrillin Antibody, clone 11C1.3 is an antibody against Fibrillin for use in ELISA, IF, IP, WB, IH(P).
Immunoblot: antibody reacts with 350,000 Da protein released from bovine aortic smooth muscle cells, which is identical to that of fibrillin.

Immunohistochemistry: fresh frozen tissue: 1:200-1:500. Paraffin sections reactive after microwave citrate buffer antigen retrieval.

Immunoprecipitation: 5 microliters of antibody diluted in no more than 400 microliters of concentrated supernatants from bovine smooth muscle cells. We recommend either an anti-mouse IgG bead or a rabbit anti-mouse capture antibody followed by protein A.

ELISA

Optimal dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

forma física

Format: Purified
Liquid

Armazenamento e estabilidade

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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The topography of microstructured surfaces differently affects fibrillin deposition by blood and lymphatic endothelial cells in culture.
Antonella Rossi, Daniela Pasqui, Rolando Barbucci, Renato Gerli, Elisabetta Weber et al.
Tissue Engineering: Part A null
Rachel Kinsey et al.
Journal of cell science, 121(Pt 16), 2696-2704 (2008-07-26)
Newly deposited microfibrils strongly colocalise with fibronectin in primary fibroblasts. Microfibril formation is grossly inhibited by fibronectin depletion, but rescued by supplementation with exogenous cellular fibronectin. As integrin receptors are key determinants of fibronectin assembly, we investigated whether they also
ADAMTS10 protein interacts with fibrillin-1 and promotes its deposition in extracellular matrix of cultured fibroblasts.
Kutz, WE; Wang, LW; Bader, HL; Majors, AK; Iwata, K; Traboulsi, EI; Sakai, LY; Keene, DR; Apte, SS
The Journal of Biological Chemistry null
Nicholas Hatzirodos et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 25(7), 2256-2265 (2011-03-18)
Although not often discussed, the ovaries of women with polycystic ovary syndrome (PCOS) show all the hallmarks of increased TGF-β activity, with increased amounts of fibrous tissue and collagen in the ovarian capsule or tunica albuginea and ovarian stroma. Recent
Placental extracellular matrix: gene expression, deposition by placental fibroblasts and the effect of oxygen.
C-P Chen, J D Aplin, C-P Chen, J D Aplin
Placenta null

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