MAB3446
Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9
ascites fluid, clone 2HP-2G9, Chemicon®
Sinônimo(s):
HP1a, Antigen p25, Chromobox Protein Homolog 5
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
ascites fluid
tipo de produto de anticorpo
primary antibodies
clone
2HP-2G9, monoclonal
reatividade de espécies
mouse, human
fabricante/nome comercial
Chemicon®
técnica(s)
ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG1
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... CBX5(23468)
Descrição geral
Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Especificidade
Reacts with Heterochromatin protein-1 alpha (HP1alpha) between amino acids 67 and 119.
Imunogênio
Recombinant mouse HP1alpha.
Aplicação
Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9 is a Mouse Monoclonal Antibody for detection of Heterochromatin Protein-1 α also known as HP1a, Antigen p25, Chromobox Protein Homolog 5 & has been validated in ELISA, IP & WB.
ELISA: 1:500-1:5,000
Western blot: 1:500-1:5,000
Immunoprecipitation: 1:500-1:5,000
Immunohistochemistry/Immunocytochemistry: Not Recommended
Note: for immunocytochemistry and immunohistochemistry we recommend CHEMICON catalog number MAB3584.
Optimal working dilutions must be determined by the end user.
Western blot: 1:500-1:5,000
Immunoprecipitation: 1:500-1:5,000
Immunohistochemistry/Immunocytochemistry: Not Recommended
Note: for immunocytochemistry and immunohistochemistry we recommend CHEMICON catalog number MAB3584.
Optimal working dilutions must be determined by the end user.
Descrição-alvo
25 kDa
forma física
Liquid ascites fluid containing no preservative.
Nota de análise
Control
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Ferramenta de seleção de produtos.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Up-regulation of Rac GTPase activating protein 1 is significantly associated with the early recurrence of human hepatocellular carcinoma.
Wang SM, Ooi LL, Hui KM
Clinical cancer research : an official journal of the American Association for Cancer Research null
Akinori Takahashi et al.
Experimental cell research, 315(7), 1117-1141 (2009-02-28)
Src-family kinases (SFKs), which participate in various signaling events, are found at not only the plasma membrane but also several subcellular compartments, including the nucleus. Nuclear structural changes are frequently observed during transcription, cell differentiation, senescence, tumorigenesis, and cell cycle.
Aurora-B/AIM-1 regulates the dynamic behavior of HP1alpha at the G2-M transition.
Terada, Y
Molecular Biology of the Cell null
A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1.
Obuse, C; Iwasaki, O; Kiyomitsu, T; Goshima, G; Toyoda, Y; Yanagida, M
Nature Cell Biology null
A role for antibodies to human leukocyte antigens, collagen-V, and K-?1-Tubulin in antibody-mediated rejection and cardiac allograft vasculopathy.
Nath DS, Tiriveedhi V, Basha HI, Phelan D, Moazami N, Ewald GA, Mohanakumar T.
Transplantation null
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica