MAB2077Z
Anti-Integrin αVβ6 Antibody
CHEMICON®, mouse monoclonal, 10D5
Sinônimo(s):
MAB2077
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
Nome do produto
Anti-Integrin αVβ6 Antibody, clone 10D5, azide free, clone 10D5, Chemicon®, from mouse
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
10D5, monoclonal
reatividade de espécies
human, rat, mouse
fabricante/nome comercial
Chemicon®
técnica(s)
flow cytometry: suitable
immunoprecipitation (IP): suitable
Isotipo
IgG2a
adequação
not suitable for Western blot
not suitable for immunohistochemistry
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... ITGAV(3685)
Descrição geral
The integrin family of cell adhesion receptors consists of at least 19 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. To date, 16 different alpha-subunits and 8 different beta-subunits have been identified. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. Integrin alphaV is a type I membrane glycoptroein with no I (inserted) domain. The alphaV protein is post-translationally cleaved into a heavy and light chain, which is expressed on the cell surface as a disulfide linked polypeptide.
The integrin alphavbeta6 is expressed on a variety of epithelial cells during dynamic processes including organogenesis, tissue injury and malignant transformation.
The integrin alphavbeta6 is expressed on a variety of epithelial cells during dynamic processes including organogenesis, tissue injury and malignant transformation.
Especificidade
Monoclonal antibody MAB2077Z, raised by immunizing beta6 knockout mice with wild type murine keratinocytes, recognizes human and murine alphaVbeta6 by flow cytometry and immunoprecipitation and has blocking activity against murine and human alphaVbeta6. 100% inhibition of alphaVbeta6-mediated cell adhesion and cell migration are achieved at a concentration of <10 μg/mL. Because the antibody was raised in knockout mice, it is likely to recognize the receptor in other species, however it has been evaluated only in human and mouse.The antibody is specific for alphaVbeta6 and does not recognize aV or other aV integrins.
Imunogênio
Immunization of beta 6 knockout mice with wild type murine keratinocytes.
Aplicação
Anti-Integrin αVβ6 Antibody, clone 10D5, azide free is an antibody against Integrin αVβ6 for use in FC & IP.
Flow cytometry: Effective for mouse and human.
Immunoprecipitation: Effective; however because this antibody is an IgG2a, protein G or rabbit anti-mouse IgG is helpful for capture
Function blocking: 100% inhibition of alphaVbeta6-mediated cell adhesion and cell migration are achieved at a concentration of 100 μg/mL.
Not effective for western blot or immunohistochemistry.
Optimal working dilutions must be determined by end user.
Immunoprecipitation: Effective; however because this antibody is an IgG2a, protein G or rabbit anti-mouse IgG is helpful for capture
Function blocking: 100% inhibition of alphaVbeta6-mediated cell adhesion and cell migration are achieved at a concentration of 100 μg/mL.
Not effective for western blot or immunohistochemistry.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
forma física
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives.
Protein A purified
Armazenamento e estabilidade
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Nota de análise
Control
HT-29 colon carcinoma cells
HT-29 colon carcinoma cells
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Não está encontrando o produto certo?
Experimente o nosso Ferramenta de seleção de produtos.
recomendado
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Jeremy K Brown et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 54(7), 807-816 (2006-03-07)
Evolved functions of integrin-alpha(v)beta(6) include roles in epithelial cell-extracellular matrix protein interactions and in the binding and activation of latent TGF-beta(1). Integrin-alpha(v)beta(6) is also exploited as a receptor by foot-and-mouth disease virus (FMDV) and may play a significant role in
Conversion of a tumor-binding peptide identified by phage display to a functional chimeric T cell antigen receptor.
Pameijer, CR; Navanjo, A; Meechoovet, B; Wagner, JR; Aguilar, B; Wright, CL; Chang et al.
Cancer Gene Therapy null
Y K Stella Man et al.
PloS one, 8(8), e70452-e70452 (2013-08-21)
We have developed a structurally-guided scaffold phage display strategy for identification of ligand mimetic bio-therapeutics. As a proof of concept we used the ligand of integrin αvβ6, a tumour cell surface receptor and a major new target for imaging and
Phenotypic and genetic characterization of a novel phenotype in pigs characterized by juvenile hairlessness and age dependent emphysema.
Bruun, CS; J?rgensen, CB; Bay, L; Cirera, S; Jensen, HE; Leifsson, PS; Nielsen et al.
BMC Genomics null
Chemically programmed antibodies targeting multiple alpha(v) integrins and their effects on tumor-related functions in vitro.
Goswami, RK; Bajjuri, KM; Forsyth, JS; Das, S; Hassenpflug, W; Huang, ZZ; Lerner et al.
Bioconjugate Chemistry null
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica