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Merck
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Key Documents

MAB2035

Sigma-Aldrich

Anti-Aggrecan Antibody

CHEMICON®, mouse monoclonal, MK-302

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

product name

Anti-Chondroitin 6 Sulfate Antibody, clone MK-302, ascites fluid, clone MK-302, Chemicon®

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

ascites fluid

tipo de produto de anticorpo

primary antibodies

clone

MK-302, monoclonal

reatividade de espécies

canine, sheep, rat, rabbit, mouse, bovine, human

fabricante/nome comercial

Chemicon®

técnica(s)

ELISA: suitable
radioimmunoassay: suitable
western blot: suitable

Isotipo

IgG1

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

dry ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... ACAN(176)

Especificidade

Reacts with a wide range of cartilage proteoglycans after either chondroitinase (ABC or AC) or testicular hyaluronidase digestion. Native proteoglycans do not react with MAB2035. Antibody binding to epitope is successfully inhibited by disaccharides of chondroitin-6-sulfate. Recognizes the chondroitin-6-sulfate stubs (preferentially in clusters) of high density cartilage proteoglycans of different species. No cross-reactivity with dermatan sulfate, keratan sulfate or chondroitin-4-sulfate.

Imunogênio

Chondroitinase ABC-digested adult human aggrecan.

Aplicação

Anti-Chondroitin 6 Sulfate Antibody, clone MK-302 is an antibody against Chondroitin 6 Sulfate for use in ELISA, RIA & WB.
ELISA at 1:100-1:5,000

RIA at 1:100-1:200.

Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity. {0.5U/mL in 100mM Tris-HCL, 30 minutes room temperature} A dramatic proteolytic digestion of the core protein (e.g. with papain or pronase) significantly reduces the antibody reactivity.

Optimal working dilutions must be determined by the end user.

forma física

Ascites liquid; no preservative.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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