MAB1568
Anti-Calretinin Antibody, clone 6B8.2
clone 6B8.2, Chemicon®, from mouse
Sinônimo(s):
calbindin 2, calbindin 2, (29kD, calretinin)
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
6B8.2, monoclonal
reatividade de espécies
mouse, rat
fabricante/nome comercial
Chemicon®
técnica(s)
immunohistochemistry: suitable
western blot: suitable
Isotipo
IgG1
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... CALB2(794)
Descrição geral
Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc
Especificidade
Calretinin is a neuron specific calcium binding protein (31 kDa) found mostly in the CNS and retina. It is present in subsets of neurons throughout the brain and spinal cord, including sensory ganglia. It shares about 55% amino acid homology with calbindin, another calcium binding protein found in brain and periphery. Studies suggest that calretinin, like calbindin, may be neuroprotective.
Other species have not been tested.
Imunogênio
Recombinant rat calretinin (Heinze, L., 2007).
Aplicação
Anti-Calretinin Antibody, clone 6B8.2 detects level of Calretinin & has been published & validated for use in IH & WB.
Immunohistochemistry:
On rat brain tissue:
IFA: 1:1,000-1:5,000
PAP: 1:1,000-1:5,000
The antibody has been used successfully on formalin-fixed, paraffin embedded human tissue after pre-treatment of the tissue slides with citrate buffer and heat treatment. Suggested starting dilution 1:100-1:400. Proteolytic digestion with pepsin is detrimental to the immunostaining.
Western blot:
1:1,000-1:3,000
Optimal working dilutions must be determined by the end user.
On rat brain tissue:
IFA: 1:1,000-1:5,000
PAP: 1:1,000-1:5,000
The antibody has been used successfully on formalin-fixed, paraffin embedded human tissue after pre-treatment of the tissue slides with citrate buffer and heat treatment. Suggested starting dilution 1:100-1:400. Proteolytic digestion with pepsin is detrimental to the immunostaining.
Western blot:
1:1,000-1:3,000
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Qualidade
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Calretinin on 10 μg of mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Calretinin on 10 μg of mouse brain lysates.
Descrição-alvo
31 kDa
forma física
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Armazenamento e estabilidade
Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
Nota de análise
Control
Rat brain cortex, Mesothelioma tissue, mouse brain lysate.
Rat brain cortex, Mesothelioma tissue, mouse brain lysate.
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Descrição
Preços
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Postnatal development of a segmental switch enables corticospinal tract transmission to spinal forelimb motor circuits.
Chakrabarty S, Martin JH
The Journal of Neuroscience null
N L Brown et al.
Development (Cambridge, England), 128(13), 2497-2508 (2001-08-09)
The vertebrate retina contains seven major neuronal and glial cell types in an interconnected network that collects, processes and sends visual signals through the optic nerve to the brain. Retinal neuron differentiation is thought to require both intrinsic and extrinsic
J C Alvarado et al.
Neuroscience, 136(3), 957-969 (2005-12-14)
In the present study, unilateral cochlear ablations were performed in adult ferrets in order to determine whether an upregulation of the calretinin immunostained plexus in the central nucleus of the inferior colliculus occurs and if so, what the time course
Developmental dynamics of piriform cortex.
Sarma, AA; Richard, MB; Greer, CA
Cerebral Cortex (1991)
Igor Jakovcevski et al.
Cerebral cortex (New York, N.Y. : 1991), 21(8), 1771-1782 (2010-12-09)
Cortical γ-aminobutyric acid (GABA)ergic interneurons in rodents originate mainly in ventrally positioned ganglionic eminences (GEs), but their origin in primates is still debated. We studied human fetal forebrains during the first half of gestation (5-23 gestational weeks, gw) for the
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