IM27L
Anti-Cathepsin B (Ab-1) Mouse mAb (CA10)
lyophilized, clone CA10, Calbiochem®
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.43
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified antibody
tipo de produto de anticorpo
primary antibodies
clone
CA10, monoclonal
forma
lyophilized
não contém
preservative
reatividade de espécies
porcine, human, bovine
não deve reagir com
rat
fabricante/nome comercial
Calbiochem®
condição de armazenamento
OK to freeze
Isotipo
IgG2a
Condições de expedição
ambient
temperatura de armazenamento
2-8°C
Informações sobre genes
mouse ... Ctsb(13030)
Descrição geral
Anti-Cathepsin B (Ab-1), mouse monoclonal, clone CA10, recognizes the ~43 kDa latent and ~31 kDa active forms of cathepsin B in HepG2 cells. It is validated for WB, IF, and IP.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/O-NS1 plasmacytoma cells. Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B.
Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B in HepG2 cells.
Imunogênio
Epitope: within the heavy chain of mature cathepsin B
Human
purified human liver cathepsin B
Aplicação
Immunoblotting (250 ng/ml)
Immunoprecipitation (1 µg/ml)
Immunofluorescence (see comments)
Immunoprecipitation (1 µg/ml)
Immunofluorescence (see comments)
Advertência
Toxicity: Standard Handling (A)
forma física
Lyophilized from 20 mM ammonium bicabonate buffer, 100 µg BSA (100 µg size) or 20 µg BSA (20 µg size).
Reconstituição
Resuspend the lyophilized antibody with 1 ml sterile PBS. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product. Avoid freeze/thaw cycles.
Nota de análise
Positive Control
Hep G2 cells
Hep G2 cells
Outras notas
Sloane, B.F. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1:137-152.
Barret, A.J. 1987. Trends Biochem. Sci.12, 193.
Chan, S.J., et al. 1986. Proc. Natl. Acad. Sci. USA83, 7721.
Barrett, A.J. and Kirschke, H. 1981. Meth. Enzymol.80, 535.
Barret, A.J. 1987. Trends Biochem. Sci.12, 193.
Chan, S.J., et al. 1986. Proc. Natl. Acad. Sci. USA83, 7721.
Barrett, A.J. and Kirschke, H. 1981. Meth. Enzymol.80, 535.
This antibody recognizes an epitope on the heavy chain of mature cathepsin B and therefore detects both mature and pro-cathepsin B. Also reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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