HSTCMAG-28SK

MILLIPLEX^® Human High Sensitivity T Cell Panel - Immunology Multiplex Assay

Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

• Sinônimo(s): Human high-sensitivity cytokine multiplex kit, Luminex^® human high-sensitivity cytokine immunoassay, Millipore human high-sensitivity cytokine panel
• Código UNSPSC: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

Propriedades

• Nível de qualidade: 200
• reatividade de espécies: human
• fabricante/nome comercial: Milliplex^®
• assay range: accuracy: 98-107%; sensitivity: 0.11-8.17 pg/mL; (Overnight Incubation minDC); standard curve range: 0.18-750 pg/mL; (IL-6); standard curve range: 0.24-1,000 pg/mL; (IL-13); standard curve range: 0.24-1,000 pg/mL; (IL-21); standard curve range: 0.31-1,250 pg/mL; (IL-8); standard curve range: 0.31-1,250 pg/mL; (MIP-1α); standard curve range: 0.37-1,500 pg/mL; (IL-7); standard curve range: 0.45-1,750 pg/mL; (TNFα); standard curve range: 0.49-2,000 pg/mL; (IL-1β); standard curve range: 0.49-2,000 pg/mL; (IL-12 (p70)); standard curve range: 0.49-2,000 pg/mL; (IL-2); standard curve range: 0.49-2,000 pg/mL; (IL-5); standard curve range: 0.61-2,500 pg/mL; (IFNγ); standard curve range: 0.61-2,500 pg/mL; (MIP-3α); standard curve range: 0.73-3,000 pg/mL; (IL-17A); standard curve range: 0.92-3,750 pg/mL; (MIP-1β); standard curve range: 1.22-5,000 pg/mL; (GM-CSF); standard curve range: 1.46-6,000 pg/mL; (IL-10); standard curve range: 1.46-6,000 pg/mL; (ITAC); standard curve range: 1.83-7,500 pg/mL; (IL-4); standard curve range: 18.3-75,000 pg/mL; (Fractalkine); standard curve range: 7.93-32,500 pg/mL; (IL-23); inter-assay cv: <15%; intra-assay cv: <10%
• técnica(s): multiplexing: suitable
• método de detecção: fluorometric (Luminex xMAP)
• Condições de expedição: wet ice

Descrição

Descrição geral

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process. Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders, and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.

The MILLIPLEX^® Human High Sensitivity T Cell Magnetic Bead Panel is to be used for the simultaneous quantification of any or all of the following analytes in human plasma, serum, and cell/tissue culture supernatant samples: Fractalkine, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-21, IL-23, ITAC, MIP-1α, MIP-1β, MIP-3α
and TNFα.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Especificidade

Cross Reactivty
There is no detectable cross-reactivity within the panel.

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Aplicação

• Analytes: Fractalkine, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-21, IL-23, I-TAC, MIP-1α, MIP-1β, MIP-3α, TNF-α
• Because there are unique features concerning sample prep and assay set-up, please read the entire protocol before running the assay.
• Recommended Sample type: Serum, plasma or tissue/cell lysate and culture supernatant
• Assay Run Time: Overnight
• Research Category: Inflammation & Immunology
• Research Subcategory: Immune Response, Metabolic Disorders, Chronic Inflammatory Disease, Cardiovascular Disease, Neuroscience

Características e benefícios

Design your multiplex kit by choosing available analytes within this panel.

Ligação

Replaces: HSTCMAG-28SK; HSCYTMAG-60SK

Armazenamento e estabilidade

Recommended storage for kit components is 2 - 8°C.

Outras notas

Please contact Technical Service for linearity of dilution.

Sensitivity: Please see kit protocol for individual assay sensitivities.

Informações legais

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Pictogramas: GHS08,GHS05,GHS07,GHS09
• Palavra indicadora: Danger
• Frases de perigo: H302 + H312 + H332,H317,H318,H373,H411
• Declarações de precaução: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Classificações de perigo: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• Órgãos-alvo: Respiratory Tract
• Código de classe de armazenamento: 10 - Combustible liquids