HFIB1-100K
MILLIPLEX® Human Fibrosis Panel 1
About This Item
Produtos recomendados
reatividade de espécies
human
fabricante/nome comercial
Milliplex®
assay range
accuracy: 95-96%
standard curve range: 10-40,000 pg/mL
(for COMP)
standard curve range: 2-10,000 pg/mL
(for IL-18BPa)
standard curve range: 6-25,000 pg/mL
(for Uteroglobin)
inter-assay cv: <20%
intra-assay cv: <10%
técnica(s)
multiplexing: suitable
método de detecção
fluorometric (Luminex® xMAP®)
Condições de expedição
wet ice
temperatura de armazenamento
2-8°C
Categorias relacionadas
Descrição geral
Our MILLIPLEX® Human Fibrosis Panel 1 is a 3-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue culture samples:
IL-18 Binding Protein A (IL-18BPa), Cartilage Oligomeric Matrix Protein (COMP), and Uteroglobin.
MILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the verification process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability).
Each MILLIPLEX® panel and kit includes:
- Quality controls (QCs) to qualify assay performance
- Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
- Optimized serum matrix to mimic native analyte environment in serum and plasma samples
- Detection antibody cocktails designed to yield consistent analyte profiles within panel
In addition each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility. The MILLIPLEX® Human Fibrosis Panel 1 thus enables you to focus on the therapeutic potential of cytokines and the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
Especificidade
Negligible or no cross-reactivity was observed between the antibodies for any one analyte and any of the other analytes in this panel.
Aplicação
- This assay may be run overnight (16-18 hours) at 2-8°C or for 2 hours at room temperature (20-25°C) with agitation on a plate shaker.
- A maximum of 25 μL per well of 1:200 serum or plasma diluted in Assay Buffer should be used for all analytes. Tissue culture supernatants may require dilution in appropriate control medium.
- Tissue/cell extracts should be prepared in neutral buffers containing reagents and conditions that do not interfere with assay performance.
Researchers will need access to a Luminex® 200, MAGPIX, or FLEXMAP 3D instrument.
Analytes Available:
- COMP
- IL-18BPa
- Uteroglobin
Research Category:
- Inflammation & Immunology
Research Sub-Category:
- Inflammation & Autoimmune Mechanisms
- Inflammation
- Infectious Diseases
- Cytokines & Cytokine Receptors
- Growth Factors & Receptors
- Oncology
- Obesity
- Metabolic Disorders
- Autoimmune disease
- Cardiovascular Disease
Características e benefícios
Design your multiplex kit by choosing available analytes within this panel.
Embalagem
Informações legais
Exoneração de responsabilidade
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Órgãos-alvo
Respiratory Tract
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
Certificados de análise (COA)
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