FCMAB113A4
Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor™ 488 conjugate
clone 10H11.2, from mouse, ALEXA FLUOR™ 488
Sinônimo(s):
Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
conjugado
ALEXA FLUOR™ 488
forma do anticorpo
purified antibody
tipo de produto de anticorpo
primary antibodies
clone
10H11.2, monoclonal
reatividade de espécies
human
técnica(s)
flow cytometry: suitable
immunocytochemistry: suitable
Isotipo
IgG1
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... POU5F1(5460)
Descrição geral
Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent embryonic stem (ES) and embryonic carcinoma (EC) cells express Oct-4. Additionally, murine embryonic germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
Especificidade
Reacts with human Oct-4.
Imunogênio
Recombinant GST fusion protein, human Oct-4 (aa 1-134).
Aplicação
Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor 488 conjugate validated for use in FC & IC.
Flow Cytometry:
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments
| Working Solution | Cells / test | Total Reaction Volume |
|---|---|---|
| 5 μl | 5 x 105 Cells / 95 μl PBS | 100 μl |
| 10 μl | 1 x 106 Cells / 90 μl PBS | 100 μl |
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
Qualidade
Evaluated by Flow Cytometry in 2102Ep Cells.
Descrição-alvo
~44 kDa
forma física
Purified mouse monoclonal IgG1 conjugated to Alexa Flour 488 in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.
Armazenamento e estabilidade
Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt
Nota de análise
Control
Human embryonic stem cells
Human embryonic stem cells
Informações legais
ALEXA FLUOR is a trademark of Life Technologies
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Shaohui Pan et al.
PloS one, 10(1), e0114423-e0114423 (2015-01-21)
Undifferentiated stem cells are better donor cells for somatic cell nuclear transfer (SCNT), resulting in more offspring than more differentiated cells. While various stem cell populations have been confirmed to exist in the skin, progress has been restricted due to
Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions.
Beers, J; Gulbranson, DR; George, N; Siniscalchi, LI; Jones, J; Thomson, JA; Chen, G
Nature Protocols null
David G Belair et al.
Scientific reports, 10(1), 2864-2864 (2020-02-20)
Exposure to thalidomide during a critical window of development results in limb defects in humans and non-human primates while mice and rats are refractory to these effects. Thalidomide-induced teratogenicity is dependent on its binding to cereblon (CRBN), the substrate receptor
Vivek Shukla et al.
Cancer research, 77(22), 6267-6281 (2017-09-25)
In this study, we generated induced pluripotent stem cells (iPSC) from normal human small airway epithelial cells (SAEC) to investigate epigenetic mechanisms of stemness and pluripotency in lung cancers. We documented key hallmarks of reprogramming in lung iPSCs (Lu-iPSC) that
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