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EZBRAIN42

Millipore

Human Amyloid β42 ELISA Kit

measures and quantifies Amyloid β42 levels in 50 μL tissue or cell lysates

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About This Item

Código UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.77

Nome do produto

Human Amyloid β42 Brain ELISA, This Human Amyloid β42 Brain ELISA is used to measure & quantify Amyloid β42 levels in Neuroscience research.

Nível de qualidade

reatividade de espécies

human

embalagem

kit of 1 × 96 wells

Parâmetros

50 μL sample volume (Overnight assay)

assay range

sensitivity: 8 pg/mL
(lowest level of Amyloid β1-42 standard; 50 μL sample size)

standard curve range: 16-500 pg/mL

técnica(s)

ELISA: suitable

entrada

sample type tissue/cellular lysate

aplicação(ões)

research use

método de detecção

colorimetric (450nm/590nm)

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Informações sobre genes

human ... APP(351)

Descrição geral

Amyloid beta peptides have been implicated in the etiology of Alzheimer’s disease. Amyloid beta 40 is the most prominent peptide and Amyloid beta 42 is the neurotoxic form. The Amyloid beta 42/40-ratio (AB ratio) has been reported as a better indicator of the Alzheimer pathology. Millipore’s Human Amyloid β42 Brain ELISA kit is used for the measurement of Amyloid beta 42 in brain samples and for other tissue samples in a 96-well format. This assay is for research use only and appropriate for the in vitro detection of human Amyloid β42 peptides in brain samples from e.g. Guinea pig, transgenic hAmyloid mice and cell extracts.

Especificidade

The Amyloid β42 ELISA uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-42, which causes a high selectivity for Aβ42. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.

Aplicação

Research Category
Neuroscience
Research Sub Category
Alzheimer′s Disease
This Human Amyloid β42 Brain ELISA is used to measure & quantify Amyloid β42 levels in Neuroscience research.
This assay requires 50 µl of sample and is an overnight assay.
Used to detect/quantify: Amyloid β42

Armazenamento e estabilidade

Components in the kit can be stored up to 2 weeks at 2-8°C

Outras notas

Please contact Technical Service for linearity of dilution.

Exoneração de responsabilidade

For research use only. Not for use in diagnostic procedures.

Pictogramas

CorrosionExclamation mark

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials


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The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(24), 8277-8288 (2014-06-13)
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Michael B McFerrin et al.
Annals of clinical and translational neurology, 4(7), 466-477 (2017-07-12)
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Y Goll et al.
Neuro-degenerative diseases, 13(2-3), 58-60 (2013-11-07)
Most Alzheimer's disease (AD) cases arise sporadically and may involve innate immune activation of microglial expressed Toll-like receptors regulated through the myeloid differentiation protein 88 (MyD88) pathway. It was the aim of this study to test the innate immune involvement
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BMC genomics, 14, 181-181 (2013-03-19)
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Molecular neurodegeneration, 10, 62-62 (2015-11-23)
L-methionine, the principal sulfur-containing amino acid in proteins, plays critical roles in cell physiology as an antioxidant and in the breakdown of fats and heavy metals. Previous studies suggesting the use of L-methionine as a treatment for depression and other

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