ESG112
ESGRO®-2i Supplement Kit (1000x)
enhances viability of mouse embryonic stem and iPS cells in the absence of serum & feeder cells
Sinônimo(s):
2i Naive ES/IPS Supplement
About This Item
Produtos recomendados
Nível de qualidade
Ensaio
>95% (active component mLIF, SDS-PAGE)
forma
liquid
fabricante/nome comercial
Chemicon®
ESGRO®
técnica(s)
cell culture | stem cell: suitable
Impurezas
<0.1 ng/μg Endotoxin (of LIF)
entrada
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
nº de adesão NCBI
nº de adesão UniProt
Informações sobre genes
human ... LIF(3976)
Descrição geral
Mouse Leukemia Inhibitory Factor (LIF) is a critical component in mouse ES cell culture media where it promotes the self-renewal and maintains the pluripotency of mouse embryonic stem (ES) cells. Recent studies have determined that inhibitors that block MAPK/Erk pathway, in combination with the glycogen synthase kinase 3 (GSK3) inhibitor, protect mouse ES cells from differentiation-inducing signals, allowing for self-renewal even in the absence of LIF. Mouse ES cells cultured in these conditions maintain their LIF responsiveness and studies have shown that adding LIF to the 2i culture conditions led better colony propagation and enhanced the general health of ES colonies. Supports a naive/ground pluripotent state.
Aplicação
1. Thaw MEK/GSK3 inhibitor supplement and mLIF at room temperature.
2. Each vial of supplement is sufficient to make 500 mL of complete medium for serum-free or serum dependent mouse ES culture.
3. Aseptically transfer the contents of the vials containing the supplements at 1000x dilution to the pre-warmed bottle of serum free basal medium such as ESGRO Complete Basal Medium (Cat. No. SF002-500) or serum containing medium and mix.
4. The complete medium can be filtered through 0.22 µm filter (Cat. No. S2GVU05RE).
5. After supplements are added to the medium, store in the dark at 4°C for up to ten days.
Embalagem
Componentes
MEK/GS3 Inhibitor Supplement: (Cat. No. CS210510-500UL): Ten (10) 0.5 mL vials.
forma física
Nota de preparo
Armazenamento e estabilidade
Nota de análise
Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL
Murine myeloid leukemic, M1 Assay: Specific Activity is greater than or equal to 108 units/mg
Informações legais
Exoneração de responsabilidade
LABEL LICENSE - USE RESTRICTED TO RESEARCH USE ONLY
Leukemia Inhibitory Factor ("LIF") is protected by US Patents No. 5,443,825 and 5,750,654 and Canada Patent No. 1,341,581 and 1,341,469 owned by AMRAD Corporation Ltd. and licensed exclusively to Millipore for research uses. The use of this product is limited for research uses only and not for any commercial purposes, such as making or developing other research products from this product or use of this product as a component in a research kit. This product is not to be used in humans or for any diagnostic purposes.
The purchaser may refuse to accept the conditions of this label license by returning this product unopened and will receive a full refund of the purchase price paid.
For-profit entities or commercial research entities purchasing this product shall be required to execute a separate commercial license with Millipore within three months of purchase. Researchers may not transfer this product or its derivatives to researchers performing commercial research at other Nonprofit Organizations, or to For-Profit Organizations, without the express written consent of Millipore, and without those entities having an express license from Millipore for the use of the product. Other than as specifically provided herein, a transferee of this product shall have no right to transfer this product, or its derivatives to any other person or entity.
Código de classe de armazenamento
10 - Combustible liquids
Certificados de análise (COA)
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Artigos
Naive pluripotent stem cells are located within the epiblast of mature blastocysts. These primitive “ground-state” cells may be cultured in vitro using specialized media and small molecule inhibitors.
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