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Key Documents

CBL180-I

Sigma-Aldrich

Anti-CD62E [ELAM-1] Antibody, clone 1.2B6

clone 1.2B6, from mouse

Sinônimo(s):

E-selectin, CD62 antigen-like family member E, CD62E, ELAM-1, Endothelial adhesion molecule 1, Endothelial leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 2, P-selectin, CD62 antigen-like family member P, CD62P, GMP-140, Granu

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

1.2B6, monoclonal

reatividade de espécies

human

técnica(s)

ELISA: suitable
activity assay: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotipo

IgG1κ

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

dry ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... SELE(6401)

Descrição geral

Selectins (CD62s) are single-chain transmembrane glycoproteins that function as cell adhesion molecules (CAMs) by interacting with a range of oligosaccharide structures including sialyl-Lewis X (sLeX) via their C-type lectin domain. All three known selectins (L-, E-, and P-selectin or CD62L CD62E, CD62P, respectively) share a similar overall domain structure that includes an N-terminal C-type lectin domain (calcium-dependent lectin domain), an EGF-like domain, a variable number of short consensus repeat (SCR) or Sushi domains (2, 6, and 9 for CD62L CD62E, CD62P, respectively), a transmembrane domain, and a cytoplasmic tail. The extracellular domains show a high homology among the three selectins, the transmembrane and cytoplasmic domains however are not conserved among the three. E-selectin (CD62E, ELAM-1) and P-selectin (CD62P, GMP-140, LECAM3, PADGEM) are mainly expressed on endothelial cells at inflammatory sites, whereas L-selectin (CD62L) is exclusively expressed on leukocytes.

Especificidade

This antibody is specific to human antigen E-selectin (ELAM-1, 115 kDa) and P-selectin that are expressed on activated endothelial cells. The ligand is expressed on neutrophils and may function as an early part in neutrophil binding to the endothelium. This antibody inhibits neutrophil adhesion to E-selectin and P-selectin when used as F(ab′)2 fragments. Although previously thought to recognize only human CD62E, later data (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560) showed that this antibody also recognizes human CD62P by binding to a common epitope on these members of the selectin family.

Aplicação

Anti-CD62E [ELAM-1] Antibody, clone 1.2B6 is an antibody against CD62E [ELAM-1] for use in Immunohistochemistry, ELISA, Flow Cytometry, Western Blotting, Activity Assay.
ELISA Analysis: A representative lot detected an induction of E-selectin expression on HUVECs by T-cells isolated from human PBMCs (Yarwood, H., et al. (2000). J. Leukoc. Biol. 68(2):233-242).
ELISA Analysis: A representative lot detected a time-dependent induction of E-selectin expression by IL-1 or TNF stimulation on HUVECs, but not A549 cells (Wellicome, S.M., et al. (1990). J.Immunol. 144(7):2558-2565).
Flow Cytometry Analysis: A representative lot specifically reacted with exogenously expressed human E- and P-, but not L-, selectin on the surface of transfected cells (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Flow Cytometry Analysis: A representative lot detected an induction of E-selectin expression by IL-1 or TNF stimulation on HUVECs (Wellicome, S.M., et al. (1990). J.Immunol. 144(7):2558-2565).
Western Blotting Analysis: A representative lot specifically reacted with E- and P-, but not L-, selectin IgG fusion proteins (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Activity Assay: A representative lot blocked the adhesion of HL-60 cells to E- or P-selectin transfected cells (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Activity Assay: A representative lot blocked sialyl-Lewis X (sLeX) binding by E- and P-selectin IgG fusion proteins (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Immunohistochemistry Analysis: A representative lot detected ELAM-1 immunoreactivity in acetone-fixed frozen human skin tissue sections (Groves, R.W., et al. (1991). Br. J. Dermatol. 124(2):117-123).
Immunoprecipitation Analysis: A representative lot immunoprecipitated E-selectin and associated proteins from lysates of TNF-stimulated HUVECs (Wellicome, S.M., et al. (1990). J.Immunol. 144(7):2558-2565).

Qualidade

Evaluated by Immunohistochemistry in human tonsil tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected CD62E [ELAM-1] in human tonsil tissue.

Descrição-alvo

66 kDa calculated.

forma física

Format: Purified

Outras notas

Concentration: Please refer to lot specific datasheet.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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