AG210
Prion Protein, recombinant
Sinônimo(s):
PrP, CD230
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.26
Produtos recomendados
fonte biológica
bovine
Nível de qualidade
Ensaio
>95% (total protein)
Formulário
liquid
fabricante/nome comercial
Chemicon®
técnica(s)
cell based assay: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
dry ice
Informações sobre genes
bovine (calf) ... PRNP(281427)
Descrição geral
Histidine-tagged full-length mature part of bovine PrP (25-244) is expressed in E. Coli BL21, solubilized from inclusion bodies in 6 M guanidine-HCl, and purified by Ni(II)-nitriloacetate agarose chromatography followed by reversed-phase HPLC (C4 column)
Prion diseases or transmissible spongiform encephalopathies are neurodegenerative diseases that affect both humans and animals (Prusiner 1998). All prion diseases share the same molecular pathogenic mechanism that involves conversion of normal cellular prion protein (PrPc) into a form that is insoluble in non ionic detergent and partially resistant to proteases (PrPSc) (Pan et al. 1993). Both PrPSc and PrPc are encoded within a single exon of a chromosomal gene as a protein of ~ 250 amino acids (Basler et al. 1986). Many mammalian PrPs have a 22 amino acid N-terminal signal sequence (Hope et al. 1986; Turk et al. 1988) and 23 amino acid C-terminal signal sequence encoding for attachment of a glycosylphosphatidylinositol anchor (Stahl et al. 1987, 1990). The mature protein of 209 amino acids contains one disulfide bond (Turk et al. 1988) and has two sites of asparagine-linked glycosylation (Endo et al. 1989; Oesch et al. 1995).
Aplicação
Antigen in standard immunochemical detection of BSE.
Optimal working dilution must be determined by the end user.
Optimal working dilution must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
forma física
Liquid in 10 mM sodium acetate buffer, pH 4.0, containing 0.01% sodium azide.
Armazenamento e estabilidade
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Nota de análise
The PrPc appears as a single band of about 27 kD by SDS-PAGE
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Purification and properties of the cellular and scrapie hamster prion proteins.
Turk, E, et al.
European Journal of Biochemistry, 176, 21-30 (1988)
Scrapie and cellular PrP isoforms are encoded by the same chromosomal gene.
Basler, K, et al.
Cell, 46, 417-428 (1986)
Prions.
Prusiner, S B
Proceedings of the National Academy of Sciences of the USA, 95, 13363-13383 (1998)
Identification of glycoinositol phospholipid linked and truncated forms of the scrapie prion protein.
Stahl, N, et al.
Biochemistry, 29, 8879-8884 (1990)
Scrapie prion protein contains a phosphatidylinositol glycolipid.
Stahl, N, et al.
Cell, 51, 229-240 (1987)
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