ABT155

Anti-Dynamin related protein 1 (Drp1) Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): Dynamin-1-like protein, Dynamin family member proline-rich carboxyl-terminal domain less, Dymple, Dynamin-related protein 1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human, mouse
• reatividade da espécie (prevista por homologia): rhesus macaque (based on 100% sequence homology), rat (based on 100% sequence homology), ox (based on 100% sequence homology)
• técnica(s): immunocytochemistry: suitable; western blot: suitable
• nº de adesão NCBI: NP_690029
• nº de adesão UniProt: Q8K1M6
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: mouse ... Dnm1L(74006)

Descrição

Descrição geral

Dynamin related protein 1 (Drp1), also known as Dynamin-like protein 1, mediates outter mitochondrial membrane fission in mammilian cells. Like other members of the Dynamin superfamily of GTPases, Drp1 contains a N-terminal tripartate GTP-binding domain and exists as a T-shaped dimer with a head, leg, and stalk. The addition of GTP induces head and stalk rearrangement resulting in membrane constriction. Drp1 has been shown to affect mitochondrial morphology and places a significant role in mitochondrial and peroxisomal fission in mammilian cells. During apoptosis, Drp1 translocates from the cytosol to mitochondria and localizes to potential organelle division sites. Studies show Drp1 inhibition averts cell death, reinforcing to the critical role that mitochondrial fission plays in apoptosis.

Especificidade

This antibody recognizes Dynamin related protein 1 (Drp1) at the GSK3B interaction domain.

Imunogênio

Epitope: GSK3B interaction domain

KLH-conjugated linear peptide corresponding to the GSK3B interaction domain of mouse Dynamin related protein 1 (Drp1).

Aplicação

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Dynamin related protein 1 in human embryonic stem cell H9 cells.

Research Sub Category
Organelle & Cell Markers

Apoptosis - Additional

This Anti-Dynamin related protein 1 (Drp1) Antibody is validated for use in WB, IC for the detection of Dynamin related protein 1 (Drp1).

Qualidade

Evaluated by Western Blot in mouse embryonic stem cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Dynamin related protein 1 (Drp1) on 10 µg of mouse embryonic stem cell lysate.

Descrição-alvo

~80kDa (isoform 2) & ~82 kDa (isoform 1) observed
5 isoforms are produced by alternative splicing: Isoform 1: 80 kDa, Isoform 2: 82 kDa, Isoform 3: 78 kDa, Isoform 4: 69 kDa, and Isoform 5: 24 kDa

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable