ABS1624

Anti-Lunapark Antibody

serum, from rabbit

• Sinônimo(s): Protein lunapark, Ulnaless
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: serum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: monkey, human
• técnica(s): western blot: suitable
• nº de adesão NCBI: NP_085153
• nº de adesão UniProt: Q9C0E8
• Condições de expedição: dry ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... KIAA1715(80856)

Descrição

Descrição geral

Protein lunapark (UniProt Q9C0E8; also known as Ulnaless) is encoded by the LNP (also known as KIAA1715) gene (Gene ID 80856) in human. The endoplasmic reticulum (ER) consists of a polygonal network of sheets and tubules interconnected by three-way junctions that undergoes continual remodeling through two competing processes. The branching and fusion of tubules lead to the formation of new three-way junctions and polygons, while junction sliding and ring closure lead to polygon loss. Both yeast Lnp1 and mammalian lunapark (mLnp1) are reported to play an important role in the maintenance of ER morphology through junctions stabilization. Study conducted in COS-7 cells demonstrated that junctions with lunapark are less mobile than junctions lacking lunapark. New junctions acquired lunapark tend to remain stable within the ER network, whereas the nascent junctions that fail to acquire lunapark preferentially undergo ring closure.

Especificidade

Exprected to react with all four spliced isoforms of human lunapark reported by UniProt (Q9C0E8).

Imunogênio

GST-tagged full-length recombinant human Lunapark.

Aplicação

Detect Lunapark using this Anti-Lunapark Antibody validated for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Western Blotting Analysis: The purified antibody was used to detect Lunapark expression in COS-7 cells and confirm siRNA-medidated Lunapark downregulation (Chen, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(2):418-423).

Qualidade

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Lunapark in 10 µg of HeLa cell lysate.

Descrição-alvo

~47 kDa observed. 47.74 kDa (isoform 1), 47.09 kDa (isoform 2), 33.01 kDa (isoform 3), and 50.85 kDa (isoform 4) calculated.

forma física

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable