ABN66

Anti-Cholesterol acyltransferase 1 Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): Sterol O-acyltransferase 1, Acyl-coenzyme A:cholesterol acyltransferase 1, ACAT-1, Cholesterol acyltransferase 1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: rhesus macaque, human
• reatividade da espécie (prevista por homologia): rhesus monkey (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rat (based on 100% sequence homology)
• técnica(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• nº de adesão NCBI: NP_003092
• nº de adesão UniProt: P35610
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... SOAT1(6646)

Descrição

Descrição geral

Cholesterol acyltransferase 1 (ACAT1) is mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA and plays a significant role in ketone body metabolism. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. Recent studies suggests that ACAT1 may be a therapeutic target for treating certain forms of Alzeheimer′s disease.

Especificidade

Other homologies: Mouse (93% sequence homology).

Imunogênio

KLH-conjugated linear peptide corresponding to human Cholesterol acyltransferase 1.

Aplicação

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Cholesterol acyltransferase 1 in human adrenal gland and normal human brain tissues.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

This Anti-Cholesterol acyltransferase 1 Antibody is validated for use in WB, IH(P) for the detection of Cholesterol acyltransferase 1.

Qualidade

Evaluated by Western Blot in Caco-2 cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected Cholesterol acyltransferase 1 on 10 µg of Caco-2 cell lysate.

Descrição-alvo

~58 kDa observed which may be the result of dimerization/aggregation of the native protein during sample preparation for SDS-PAGE. The calculated molecular weight is 65 kDa

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Caco-2 cell lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable