ABE991

Anti-NeuroD1 Antibody

serum, from rabbit

• Sinônimo(s): Neurogenic differentiation factor 1, NeuroD1, Beta-cell E-box transcriptional activator 2, Beta2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: serum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: rat, mouse
• técnica(s): ChIP: suitable; immunohistochemistry: suitable; western blot: suitable
• nº de adesão NCBI: NP_035024
• nº de adesão UniProt: Q60867
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: mouse ... Neurod1(18012) ; rat ... Neurod1(29458)

Descrição

Descrição geral

NeuroD1 is a transcriptional activator that associates with p300 transcription co-activator protein to promote the expression of many important differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. NeuroD1 is required for amacrine cell fate as well as dendrite morphogenesis and the maintenance of cerebellar cortex. Also via its associate with chromatin, NeuroD1 helps regulate the expression of many other key transcriptional regulators of neurogenesis as well. NeuroD1 is expressed in developing brain and pancreas as well as in differentiating neurons in both the CNS and PNS systems. Disruption of NeuroD1 leads to early death in mice with several developmental and functional defects throughout the body pointing to this factors key role in development in many systems.

Imunogênio

GST-tagged recombinant protein corresponding to mouse NeuroD1.

Aplicação

Immunohistochemistry Analysis: A representative lot detected NeuroD1 in developing pituitary tissue (Lavoie, P., et al. (2008). Molecular Endocrinology. 22:1647-1657).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated NeuroD1 in AtT-20 cell lysate (Langlais, D., et al. (2011). Molecular Endocrinology. 25:348-359).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-NeuroD1 Antibody is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and Immunohistochemistry for the detection of NeuroD1.

Qualidade

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NeuroD1 in 10 µg of rat brain tissue lysate.

Descrição-alvo

~40 kDa observed. Uncharacterized band(s) may appear in some lysates.

forma física

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1