ABE284

Anti-T-box transcription factor TBX5 Antibody

serum, from rabbit

• Sinônimo(s): T-box transcription factor TBX5, T-box protein 5
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: serum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: mouse, rat, human
• técnica(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• nº de adesão NCBI: NP_035667
• nº de adesão UniProt: P70326
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... TBX5(6910)

Descrição

Descrição geral

The gene encoding T-box transcription factor 5 (TBX5) is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity.

Imunogênio

Recombinant protein corresponding to mouse T-box transcription factor TBX5.

Aplicação

Anti-T-box transcription factor TBX5 Antibody detects level of T-box transcription factor TBX5 & has been published & validated for use in Western Blotting, ICC & IHC.

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected T-box transcription factor TBX5 in 293T cells overexpressing T-box transcription factor TBX5, mouse primary cardiocytes treated with serum, HA-tagged Tbx5 transfected NIH/3T3 cells, and HA-tagged Tbx5 transfected C2C12 cells (Georges, R., et al. (2008). Mol Cell Biol. 28(12):4052-4067.)

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected T-box transcription factor TBX5 in mouse embryonic tissue and in Tbx5 transgenic mouse heart tissues treated with tamoxifin (Georges, R., et al. (2008). Mol Cell Biol. 28(12):4052-4067.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Qualidade

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:1,000 dilution from a representative lot detected T-box transcription factor TBX5 in 10 µg of HeLa cell lysate.

Descrição-alvo

~53 kDa observed. An uncharacterized band may be observed at ~75 kDa in some cell lysates. Three isoforms due to alternative splicing may be observed at ~35 kDa, ~50 kDa, and ~64 kDa in some cell lysates. (Georges, R., et al. (2008). Mol Cell Biol. 28(12):4052-4067.).

forma física

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análise

Control
HeLa cell lysate

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1