ABD121

Anti-PRDM14 Antibody

from rabbit

• Sinônimo(s): PRDM-14, PR domain zinc finger protein 14
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: unpurified
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: human
• técnica(s): ChIP: suitable (ChIP-seq); western blot: suitable
• Isotipo: IgG
• nº de adesão UniProt: Q9GZV8
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PRDM14(63978)

Descrição

Descrição geral

PRDM14 is an approximately 65 kDa transcriptional inhibitor that contains one histone methylating PR/SET domain (aa 266 - 367) and six zinc finger repeats (aa 400 - 568). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is upregulated in breast cancer and lymphoblastic leukemia. Recently, PRDM14 ensures naive pluripotency through dual regulation of signaling and epigenetic pathways in embryonic stem cells

Especificidade

Human

Imunogênio

Epitope: N-terminus

Human recombinant PRDM14.

Aplicação

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory on H1 human ES cells (CHAN et al., 2013. STEM CELLS, 31:682–692)

ChIPseq Analysis: A representative lot was used by an independent laboratory on H1 human ES cells (Chia et al., 2010. Nature. 316, VOL 468)

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

This Anti-PRDM14 Antibody is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of PRDM14 .

Qualidade

Evaluated by Western Blotting in Human ES cell cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected PRDM14 in undifferentiated human ES cell lysate.

Descrição-alvo

~64 kDa observed. Uncharacterized bands may be observed in some lysate(s).

forma física

Format: Unpurified

Raw rabbit polyclonal serum

Unpurified

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable